Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen

Sawhney, S.M.S. and Hasima, N.N. and Glass, E.J. and Almurrani, S.W.K. and Nichani, A.K. and Spooner, R.L. and Williams, J.L. and Russell, G.C. (1995) Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen. Immunogenetics, 41 (4). pp. 246-250. ISSN 0093-7711,

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Cattle major histocompatibility complex (MHC) (BoLA) class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8{sup +} T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been characterized mainly by using allo-antisera raised by reciprocal calf/dam immunizations. This has enabled the identification of about 50 serlogical specificities, most of which behave as alleles of a single highly polymorphic class I locus. However, evidence from biochemical and molecular biological studies suggest that more than one BoLA class I locus is expressed. These loci are apparently in linkage disequilibrium, making them difficult to distinguish by conventional methods. In order to investigate the expression and function of individual class I locus products, we are correlating BoLA class I gene sequences with the expressed products by the transfection and characterization of genomic class I clones. Shotgun transfection and expression of BoLA class I molecules has been described previously, but the genes involved were not isolated. In this paper we report the isolation, DNA sequencing, transfection, and expression of a genomic clone encoding a BoLA-A11 determinant from an animal expressing A10 and A11 serological specificities. 23 refs., 3 figs.

Item Type: Article
Uncontrolled Keywords: Amino acid sequence; Animals; Base sequence; Cattle; Gene expression; Genetics; Genes; Molecular sequence data; Transfection
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 11 Jul 2013 03:28
Last Modified: 15 Nov 2013 02:58

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