A study on the energy source in the developing embryo of the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille)

Srijaya, T.C. and Pradeep, P.J. and Shaharom, F. and Chatterji, A. (2012) A study on the energy source in the developing embryo of the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille). Invertebrate Reproduction & Development, 56 (4). pp. 305-314. ISSN 0792-4259, DOI https://doi.org/10.1080/07924259.2011.633621.


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The mangrove horseshoe crab, Carcinoscorpius rotundicauda, is an Asian species found in Malaysia. This ancient arthropod has a long incubational period during which it depends on various energy sources for both embryogenesis and organogenesis. This study describes the trend of energy utilization from the endogenous reserves by the developing embryos from 0 to 40 days of incubation (until the hatching of the larvae). The dry weight, insoluble protein, carbohydrate, glycogen and lipid showed a declining trend from 0 to 40 days of incubation, whereas the wet weight, water content, ash content and soluble protein showed an increasing trend. Selected micro-elements such as Cu2+, Fe2+ and Zn2+ also demonstrated an interesting trend in the developing eggs when the egg mass was subjected to inductively coupled plasma mass spectrometry analysis, where these elements showed a high correlation with the moulting stages and egg development. Maximum variations within the micro-elements were observed during the 1st (10 days after fertilization) and 2nd (35-36 days after fertilization) moulting stages within the developing eggs. This study clearly indicated that the moulting cycles of C. rotundicauda during embryonic development influence energy utilization in the form of carbohydrates, lipids, proteins, glycogen and other micro-elements.

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Additional Information: ISI Document Delivery No.: 051IP Times Cited: 0 Cited Reference Count: 67 Cited References: AOAC, 1990, OFFICIAL METHODS ANA, VII BABU DE, 1987, MAR BIOL, V95, P123, DOI 10.1007/BF00447493 BARNES H, 1965, J MAR BIOL ASSOC UK, V45, P321 Biesiot PM, 1995, MAR BIOL, V124, P407, DOI 10.1007/BF00363914 Botton ML, 2000, B ENVIRON CONTAM TOX, V64, P137, DOI 10.1007/s001289910021 Botton ML, 1998, ARCH ENVIRON CON TOX, V35, P25 Bryan PJ, 2004, MAR BIOL, V145, P293, DOI 10.1007/s00227-004-1311-0 Buckup L, 2008, COMP BIOCHEM PHYS A, V149, P59, DOI 10.1016/j.cbpa.2007.10.008 Burger J, 1997, ENVIRON MONIT ASSESS, V46, P279, DOI 10.1023/A:1005718419708 Burger J, 2002, ENVIRON RES, V90, P227, DOI 10.1016/S0013-9351(02)00027-0 Chatterji Anil, 1996, Journal of Aquaculture in the Tropics, V11, P271 Chatterji A., 1994, ORISSA INDIA A PROJE, P157 Coutteau P, 1997, AQUACULTURE, V155, P149, DOI 10.1016/S0044-8486(97)00125-7 Dreon MS, 2006, BIOCELL, V30, P359 DUBOIS M, 1956, ANAL CHEM, V28, P350, DOI 10.1021/ac60111a017 FOLCH J, 1957, J BIOL CHEM, V226, P497 GALLAGER SM, 1986, AQUACULTURE, V56, P81, DOI 10.1016/0044-8486(86)90020-7 Garcia-Guerrero M, 2003, J CRUSTACEAN BIOL, V23, P1, DOI 10.1651/0278-0372(2003)0230001:VILPAC2.0.CO;2 Gardner C, 2001, MAR FRESHWATER RES, V52, P333, DOI 10.1071/MF98027 George SB, 1999, J EXP MAR BIOL ECOL, V237, P203, DOI 10.1016/S0022-0981(98)00205-6 Giannenas I, 2009, FOOD CHEM, V114, P706, DOI 10.1016/j.foodchem.2008.09.079 Gimenez L, 2005, J PLANKTON RES, V27, P485, DOI 10.1093/plankt/fbi024 Gimenez L, 2001, J EXP MAR BIOL ECOL, V260, P241, DOI 10.1016/S0022-0981(01)00258-1 HoeghGuldberg O, 1997, BIOL BULL, V192, P27, DOI 10.2307/1542573 Holland DL, 1978, BIOCH BIOPHYSICAL PE, P85 Itow T, 1998, ARCH ENVIRON CON TOX, V35, P457 Itow T, 1998, ARCH ENVIRON CON TOX, V35, P33 JAECKLE WB, 1989, MAR BIOL, V103, P87, DOI 10.1007/BF00391067 KAVANAU JL, 1954, EXP CELL RES, V7, P530, DOI 10.1016/S0014-4827(54)80097-8 Laughlin JR, 1981, J EXPT MARINE BIOL E, V52, P135 Lavolpe M, 2004, ENVIRON TOXICOL CHEM, V23, P960, DOI 10.1897/02-645 Lee R.F., 1991, Advances in Comparative and Environmental Physiology, V7, P187 Leong PKK, 1999, J EXP BIOL, V202, P2051 Marsh AG, 2001, SCIENCE, V291, P1950, DOI 10.1126/science.1056341 Marsh AG, 1999, J EXP BIOL, V202, P2041 Moran AL, 2009, BIOL BULL-US, V216, P226 Moran AL, 2003, BIOL BULL-US, V204, P270, DOI 10.2307/1543598 NEEDHAM A. E., 1957, PHYSIOL COMPARATA ET OECOL, V4, P209 Paez-Osuna F, 1998, MAR POLLUT BULL, V36, P65, DOI 10.1016/S0025-326X(98)90035-2 Palacios E, 1999, AQUACULTURE, V171, P209 Pandian TJ, 1967, HELGOLAND MARINE RES, V16, P193 Pandian TJ, 1970, MAR BIOL, V5, P153 PANDIAN TJ, 1967, HELGOLAND WISS MEER, V16, P216, DOI 10.1007/BF01611705 PANDIAN TJ, 1970, MAR BIOL, V7, P249, DOI 10.1007/BF00367495 Reppond K, 2008, J CRUSTACEAN BIOL, V28, P519, DOI 10.1651/07-2862R1.1 RODRIGUEZ JL, 1990, MAR BIOL, V106, P109, DOI 10.1007/BF02114680 Rodriguez-Gonzalez H, 2006, AQUACULTURE, V257, P412, DOI 10.1016/j.aquaculture.2006.01.020 Russo F, 1970, ZEITSCHRIFT FUR ZELL, V104, P14 Saigusa Masayuki, 1992, Pacific Science, V46, P484 Samuel N. John, 2009, International Journal of Animal and Veterinary Advances, V1, P32 Samuel NJ, 2010, CURR RES J BIOL SCI, V2, P38 Sanchez MV, 2005, ENVIRON RES, V99, P72, DOI 10.1016/j.envres.2004.09.005 Sanchez-Paz A, 2006, INSECT BIOCHEM MOLEC, V36, P241, DOI 10.1016/j.ibmb.2006.01.002 SASAKI GC, 1986, CAN J FISH AQUAT SCI, V43, P2311 SAUDRAY Y, 1954, CR SOC BIOL, V148, P814 Shakuntala K, 1972, HYDROBIOLOGIA, V40, P381 SHAKUNTALA K, 1982, INT J INVER REP DEV, V4, P381 SHAKUNTALA K, 1976, EXPERIENTIA, V32, P627, DOI 10.1007/BF01990202 Shilling FM, 1996, BIOL BULL, V191, P402, DOI 10.2307/1543013 Sibert V, 2004, MAR BIOL, V144, P1075, DOI 10.1007/s00227-003-1287-1 Srijaya T.C., 2010, Our Nature, V8, P204 Vavra J, 1999, BIOL BULL, V196, P177, DOI 10.2307/1542563 Walls EA, 2002, REV FISH SCI, V10, P39, DOI 10.1080/20026491051677 Wehrtmann IS, 1998, J CRUSTACEAN BIOL, V18, P413, DOI 10.2307/1549406 Whyte J. N. C., 1993, Aquaculture and Fisheries Management, V24, P193, DOI 10.1111/j.1365-2109.1993.tb00541.x Zaleha K., 2010, Journal of Biological Sciences, V10, P790, DOI 10.3923/jbs.2010.790.794 Zancan DM, 2010, JOURNAL OF EXPERIMEN, V313, P280 Srijaya, Thekkeparambil Chandrabose Pradeep, Padmaja Jayaprasad Shaharom, Faizah Chatterji, Anil University Malaysia Terengganu The authors are grateful to the University Malaysia Terengganu for providing SKS fellowships to Thekkeparambil Chandrabose Srijaya, Padmaja Jayaprasad Pradeep and Principal Research Fellowship to Anil Chatterji. The authors thank Mr Joseph from Institute of Oceanography for his kind help during ICP-MS analysis and Dr Mithun Sukumaran for successfully completing this study. Int science services/balaban publishers Rehovot
Uncontrolled Keywords: Arthropod trilobite larvae embryonic development biochemical composition micro-elements lobster homarus-americanus antarctic sea-urchin chasmagnathus-granulatus brachyura crayfish cherax-quadricarinatus biochemical-composition limulus-polyphemus haliotis-rufescens heavy-metals egg size lecithotrophic larvae
Subjects: R Medicine > RK Dentistry
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Faculty of Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 19 Apr 2013 01:10
Last Modified: 19 Apr 2013 01:10
URI: http://eprints.um.edu.my/id/eprint/5835

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