Molecular identification of Cryptosporidium parvum from avian hosts

Quah, J.X. and Ambu, S. and Lim, Y.A.L. and Mahdy, M.A.K. and Mak, J.W. (2011) Molecular identification of Cryptosporidium parvum from avian hosts. Parasitology, 138 (5). pp. 573-577. ISSN 0031-1820, DOI 21232175.

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Cryptosporidium species are protozoan parasites that infect humans and a wide variety of animals. This study was aimed at identifying Cryptosporidium species and genotypes isolated from avian hosts. A total of 90 samples from 37 different species of birds were collected throughout a 3-month period from April 2008 to June 2008 in the National Zoo of Kuala Lumpur, Malaysia. Prior to molecular characterization, all samples were screened for Cryptosporidium using a modified Ziehl-Neelsen staining technique. Subsequently samples were analysed with nested-PCR targeting the partial SSU rRNA gene. Amplicons were sequenced in both directions and used for phylogenetic analysis using Neighbour-Joining and Maximum Parsimony methods. Although 9 (10) samples were positive for Cryptosporidium via microscopy, 8 (8.9) produced amplicons using nested PCR. Phylogenetic trees identified all the isolates as Cryptosporidium parvum. Although C. parvum has not been reported to cause infection in birds, and the role of birds in this study was postulated mainly as mechanical transporters, these present findings highlight the significant public health risk posed by birds that harbour the zoonotic species of Cryptosporidium.

Item Type: Article
Additional Information: Quah, J. X. Ambu, S. Lim, Y. A. L. Mahdy, M. A. K. Mak, J. W.
Uncontrolled Keywords: Animals Animals, Zoo/parasitology Base Sequence Bird Diseases/epidemiology/*parasitology Birds Cryptosporidiosis/epidemiology/parasitology/*veterinary Cryptosporidium/classification/genetics/isolation & purification Cryptosporidium parvum/classification/*genetics/isolation & purification DNA, Protozoan/genetics DNA, Ribosomal/chemistry/genetics Disease Vectors Feces/parasitology Genotype Humans Malaysia/epidemiology Molecular Sequence Data Phylogeny Polymerase Chain Reaction Prevalence RNA, Ribosomal, 18S/genetics Risk Factors Sequence Analysis, DNA
Subjects: R Medicine
Divisions: Faculty of Medicine > School of Medicine
Depositing User: Ms Johana Johari
Date Deposited: 05 Dec 2012 04:03
Last Modified: 05 Dec 2012 04:03

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