Effect of Piper betle L. leaf extract on the virulence activity of Streptococcus mutans - an in vitro study

Nalina, T. and Rahim, Z.H.A. (2006) Effect of Piper betle L. leaf extract on the virulence activity of Streptococcus mutans - an in vitro study. Pakistan Journal of Biological Sciences, 9 (8). pp. 1470-1475. ISSN 10288880, DOI https://doi.org/10.3923/pjbs.2006.1470.1475.


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In this study the effect of crude aqueous extract of the leaves of Piper betle L. on the virulence properties of Streptococcus mutans ATCC 25175 was investigated. It was carried out based on the effect of the extract towards growth, cell surface hydrophobicity, adhering property and glucosyltransferase activity of the S. mutans. The concentration of crude aqueous extract of Piper betle L. used in the experiments above was between 0 to 20 mg mL-1. Chlorhexidine (0.12) and sterile deionised water was used as positive and blank control, respectively. The results obtained showed that the crude extract at a concentration as low as 2.5 mg mL-1 exhibited reduced effect towards the growth (p<0.01), adhering ability (p<0.01), glucosyltransferase activity (p<0.05) and cell surface hydrophobicity (p<0.05) of S. mutans when compared with the blank control. This implies that the Piper betle L. extract may have anti-virulence property towards S. mutans. © 2006 Asian Network for Scientific Information.

Item Type: Article
Additional Information: Cited By (since 1996): 8 Export Date: 10 September 2012 Source: Scopus Language of Original Document: English Correspondence Address: Rahim, Z.H.A.; Department of Oral Biology, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia References: Bowen, W.H., Do we need to be concerned about dental caries in the coming millennium? (2002) Cri. Rev. Oral Biol. Med., 13, pp. 126-231; Doyle, R.J., Rosenberg, M., Drake, D., Microbial Cell Surface Hydrophobicity (1990) Am. Soc. Microbiol., p. 387. , Washington. DC; Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356; Fathilah, A.R., Bakri, M.M., Rahim, Z.H.A., (2000) Pascasidang Simposium Sains Kesihatan Kebangsaan Ke-3, pp. 216-219. , Fakulti Sains Kesihatan Bersekutu, UKM; Frostell, G., Keyes, P.H., Larson, R.H., The effect of various sugars and sugar substitutes on dental caries in hamsters and rat (1967) J. Nutr., 93, pp. 65-76; Fukushima, K., Motoda, R., Takada, K., Ikeda, T., Resolution of Streptococcus mutans glucosyltransferase into two components essential to water-insoluble glucan synthesis (1981) FEBS Lett., 128, pp. 213-216; Gibbons, R.J., Van Houte, J., Dental caries (1975) Ann. Rev. Med., 26, pp. 121-136; Hamada, S., Slade, H.D., Biology, immunology and cariogenicity of Streptococcus mutans (1980) Microbiol. Rev., 44, pp. 331-384; Hamilton-Miller, J.M.T., Anti-cariogenic properties of tea (Camellia sinensis) (2001) J. Med. Microbiol., 50, pp. 299-302; Koo, M.H., Park, Y.K., Investigation of flavonoid aglycones in propolis collected by two different varieties of bees in the same region (1997) Biosci., Biotechnol. Biochem., 61, pp. 367-369; Loesche, W.J., Role of Streptococcus mutans in human dental decay (1986) Microbiol. Rev., 50, pp. 353-380; Marsh, P.D., Microbial ecology of dental plaque and its significance in health and disease (1994) Adv. Dental Res., 8, pp. 263-271; Marsh, P.D., Bradshaw, D.J., Dental plaque as a biofilm (1995) J. Ind. Microbiol., 15, pp. 169-175; Matsumoto, M., Minami, T., Sasaki, H., Sobue, S., Hamada, S., Ooshima, T., Inhibitory effects of oolong tea extract on caries inducing properties of mutans streptococci (1999) Caries Res., 33, pp. 441-445; McBride, B.C., Song, M., Krasse, B., Olsson, J., Biochemical and immunological differences between hydrophobic and hydrophilic strain of Streptococcus mutans (1984) Infection and Immun., 44, pp. 68-75; Mukasa, H., Slade, H.D., Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation (1973) Infect. Immun., 8, pp. 556-562; Nakahara, K., Kawabata, S., Ono, H., Ogura, K., Tanaka, T., Ooshima, T., Hamada, S., Inhibitory effect of oolong tea polyphenols on glucosytransferase of mutans Streptococci (1993) Applied Environ. Microbiol., 59, pp. 968-973; Nostro, A., Cannatelli, M.A., Crisafi, G., Musolino, A.D., Procopio, F., Alonzo, V., Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract (2004) Lett. Applied Microbiol., 38, pp. 423-427; Ong, H.C., Nordiana, M., Malay ethno-medico botany in Machang, Kelantan, Malaysia (1999) Fitoterapia, 70, pp. 502-513; Ooshima, T., Osaka, Y., Sasaki, H., Osawa, K., Yasuda, H., Matsumura, M., Sobue, S., Matsumoto, M., Caries inhibitory activity of cacao bean husk extract in in vitro and animal experiments (2000) Arch. Oral Biol., 45, pp. 639-645; Ponglux, D., Wong, S., Phadungcharoen, T., Ruangrungsri, N., Likhitwitaya, K., (1987) Medicinal Plants, pp. 194-209. , Victory Power Point Corp, Bangkok; Razak, F.A., Rahim, Z.H.A., The anti-adherence effect of Piper betle L. and Psidium guajava extracts on the adhesion of early settlers in dental plaque to saliva-coated glass surfaces (2003) J. Oral Sci., 45, pp. 201-206; Rosenberg, M., Gutnick, D., Rosenberg, E., Adherence of bacteria to hydrocarbons: A simple method for measuring cell surface hydrophobicity (1980) FEMS Microbiol. Lett., 9, pp. 29-33; Satou, N., Satou, J., Shintani, H., Okuda, K., Adherence of streptococci to surface-modified glass (1988) J. General Microbiol., 134, pp. 1299-1305; Slade, H.D., In vitro Models for the Study of Adherence of Oral Streptococci (1976) Immunologic Aspects of Dental Caries, pp. 21-38. , Bowen, W.H., R.J. Genco and T.C. O'Brien (Eds.), Information Retrieval Inc., Washington, DC; Weiss, E., Rosenberg, M., Judes, H., Rosenberg, E., Cell surface hydrophobicity of adherent oral bacteria (1982) Curr. Microbiol., 7, pp. 125-128; Westergren, G., Olsson, J., Hydrophobicity and adherence of oral streptococci after repeated sub culture in vitro (1983) Infect. Immun., 40, pp. 432-435
Uncontrolled Keywords: Adherence Glucosyltransferase Hydrophobicity Piper betle L. Streptococcus mutans Piper betle
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of Oral Biology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 10 Oct 2012 01:53
Last Modified: 10 Oct 2012 01:53
URI: http://eprints.um.edu.my/id/eprint/3747

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