Toxicological assessment of Opuntia dillenii (Ker Gawl.) Haw. cladode methanol extract, fractions and its alpha pyrones: Opuntiol and opuntioside

Siddiqui, Faheema and Farooq, Ahsana Dar and Mudassar, . and Kabir, Nurul and Fatima, Narjis and Abidi, Lubna and Lubna, . and Faizi, Shaheen (2021) Toxicological assessment of Opuntia dillenii (Ker Gawl.) Haw. cladode methanol extract, fractions and its alpha pyrones: Opuntiol and opuntioside. Journal of Ethnopharmacology, 280. ISSN 0378-8741, DOI

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Ethnopharmacological relevance: The edible plant Opuntia dillenii (Ker Gawl.) Haw. commonly known as Nagphana, belongs to the Cactaceae family. It is traditionally used to treat various ailments including inflammation, gastric ulcers, diabetes, hepatitis, asthma, whooping cough and intestinal spasm. Aim of the study: Despite its traditional use in various countries, detailed toxicological studies of O. dillenii cladode are few. Thus in the current study, toxicity of O. dillenii cladode derived methanol extract, fractions and its alpha-pyrones: opuntiol and opuntioside have been addressed. Methods: The test agents were assessed using both in vitro and in vivo toxicity assays. MTT on human embryonic kidney cell line (HEK-293), tryphan blue exclusion in rat neutrophils, Cytokinesis-B block micronucleus (CBMN) in human lymphocytes and genomic DNA fragmentation using agarose gel electrophoresis were performed. In acute toxicity test, mice orally received extract (5 g/kg) for 7 days followed by measurements of relative organ weight, biochemical (blood profile, liver and kidney function test) and histological studies (liver and kidney) were carried out. Rat bone marrow micronucleus genotoxicity assay was also conducted. Results: O. dillenii derived test agents were non-cytotoxic and had no effect on the integrity of DNA. Methanol extract (5 g/kg) orally administered in mice did not cause any significant change in relative organ weights, biochemical parameters and liver and kidney histology as compared to vehicle control. In parallel, extract did not stimulate micronuclei formation in rat bone marrow polychromatic erythrocytes. Conclusion: These results led to conclude that edible O. dillenii extract is non-toxic via the oral route and appears to be non-cyto-, hepato-, nephro- or genotoxic, thereby supporting its safe traditional use against various ailments. Therefore, opuntiol and opuntioside may serve as lead compounds in designing new drug(s) derived from edible plants.

Item Type: Article
Funders: Higher Education Commission of Pakistan[17-7/(179/ACADSCH/2002-128)]
Uncontrolled Keywords: Genotoxicity;Cytotoxicity;Micronucleus;DNA fragmentation;Opuntiol;Opuntioside
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
R Medicine
R Medicine > RM Therapeutics. Pharmacology
S Agriculture > SB Plant culture
Divisions: Faculty of Science
Depositing User: Ms Zaharah Ramly
Date Deposited: 22 Jun 2022 07:47
Last Modified: 22 Jun 2022 07:47

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