Dual activity of Meloidogyne incognita-regulated Musa acuminata Pathogenesis-related-10 (MaPR-10) gene

Rajendram, Arullthevan and Mostaffa, Nur Hikmah and Dumin, Walftor and Oke, Mushafau Adebayo and Simarani, Khanom and Somasundram, Chandran and Razali, Zuliana and Rejab, Nur Ardiyana and Al-Idrus, Aisyafaznim (2022) Dual activity of Meloidogyne incognita-regulated Musa acuminata Pathogenesis-related-10 (MaPR-10) gene. Gene, 809. ISSN 0378-1119, DOI https://doi.org/10.1016/j.gene.2021.146041.

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Plant immunity to pathogen infections is a dynamic response that involves multiple organelles and defence signalling systems such as hypersensitive response (HR) and systemic acquired resistance (SAR). The latter requires the function of Pathogenesis-related (PR) proteins, a common plant protein family with diverse roles in plant innate immunity. Our previous proteomics study showed that a PR gene (ITC1587_Bchr9_P26466_MUSBA) was differentially regulated during a compatible banana-M. incognita interaction, substantiating the isolation of this gene in the current study. Here, we successfully isolated and characterised Pathogenesis-related-10 (PR10) gene with beta-1,3-glucanase and ribonuclease (RNase) activities from two Musa acuminata cultivars (denoted as MaPR10) namely Berangan and Grand Naine (ITC1256). We found that MaPR10 cloned sequences possess glycine-rich loop domain and shared conserved motifs specific to PR10 gene group, confirming its identity as a member of this group. Interestingly, we also found a catalytic domain sequence for glycoside hydrolase family 16 (EXDXXE), unique only to MaPR10 cloned sequences. Two peptide variants closely related to the reference sequence ITC1587_Bchr9_P26466_MIJSBA namely MaPR10-BeB5 and MaPR10-GNA5 were overexpressed and purified to test for their functionality. Here, we confirmed that both protein variants possess beta-1,3-glucanase and ribonuclease (RNase) activities, and inhibit the growth of Aspergillus fumigatus, a human opportunistic pathogen. To our knowledge, this is the first PR10 plant proteins with such properties to be reported thus far.

Item Type: Article
Funders: Ministry of Education, Malaysia[FP035-2017A], Ministry of Education, Malaysia[PG343-2016A]
Uncontrolled Keywords: Antifungal enzyme;Aspergillus fumigates;Beta-1,3-Glucanase; Pathogenesis-related-10 gene;Meloidogyne incognita;Musa spp.;Ribonuclease
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
R Medicine
R Medicine > RB Pathology
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 25 Jul 2022 04:53
Last Modified: 25 Jul 2022 04:53
URI: http://eprints.um.edu.my/id/eprint/33686

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