Development of a novel model for the investigation of implant-soft tissue interface

Chai, W.L. and Moharamzadeh, K. and Brook, I.M. and Emanuelsson, L. and Palmquist, A. and Van Noort, R. (2010) Development of a novel model for the investigation of implant-soft tissue interface. Journal of Periodontology, 81 (8). pp. 1187-1195. ISSN 0022-3492, DOI

[img] PDF
Restricted to Registered users only

Download (3MB) | Request a copy

Download (32kB)


Background: In dental implant treatment, the long-term prognosis is dependent on the biologic seal formed by the soft tissue around the implant. The in vitro investigation of the implant-soft tissue interface is usually carried out using a monolayer cell-culture model that lacks a polarized-cell phenotype. This study developed a tissue-engineered threedimensional oral mucosal model (3D OMM) to investigate the implant-soft tissue interface. Methods: A 3D OMM was constructed using primary human oral keratinocytes and fibroblasts cultured on a skin-derived scaffold at an air-liquid interface. A titanium implant was inserted into the engineered oral mucosa and further cultured to establish epithelial attachment. The 3D OMM was characterized using basic histology and immunostaining for cytokeratin (CK) 10 and CK13. Histomorphometric analyses of the implant-soft tissue interface were carried out using a light-microscopy (LM) examination of ground sections and semi-thin sections as well as scanning electron microscopy (SEM). Results: Immunohistochemistry analyses suggests that the engineered oral mucosa closely resembles the normal oral mucosa. The LM and SEM examinations reveal that the 3D OMM forms an epithelial attachment on the titanium surface. Conclusion: The 3D OMM provided mimicking peri-implant features as seen in an in vivo model and has the potential to be used as a relevant alternative model to assess implant-soft tissue interactions.

Item Type: Article
Additional Information: Cited By (since 1996): 2 Export Date: 5 April 2012 Source: Scopus CODEN: JOPRA PubMed ID: 20450401 Language of Original Document: English Correspondence Address: Chai, W. L.; Department of General Dental Practice and Oral and Maxillofacial Imaging, University of Malaya, Kuala Lumpur 50603, Malaysia; email: Chemicals/CAS: titanium, 7440-32-6; Dental Implants; Dental Materials; Keratin-10, 147785-83-9; Keratin-13; Titanium, 7440-32-6 References: Brånemark, P.I., Hansson, B.O., Adell, R., Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period (1977) Scand. J. Plast. Reconstr. Surg. Suppl., 16, pp. 1-132; Carmichael, R., Apse, P., Zarb, G., McCulloch, C., Biological, microbiological and clinical aspects of the pre-implant mucosa (1989) The Brånemark Osseointergrated Implant, pp. 39-78. , Albrektsson T, Zarb G, eds, Chicago: Quintessence; Berglundh, T., Lindhe, J., Marinello, C., Ericsson, I., Liljenberg, B., Soft tissue reaction to de novo plaque formation on implants and teeth. An experimental study in the dog (1992) Clin. Oral. Implants Res., 3, pp. 1-8; Ericsson, I., Berglundh, T., Marinello, C., Liljenberg, B., Lindhe, J., Long-standing plaque and gingivitis at implants and teeth in the dog (1992) Clin. Oral. Implants Res., 3, pp. 99-103; Atsuta, I., Yamaza, T., Yoshinari, M., Changes in the distribution of laminin-5 during peri-implant epithelium formation after immediate titanium implantation in rats (2005) Biomaterials, 26, pp. 1751-1760; Atsuta, I., Yamaza, T., Yoshinari, M., Ultrastructural localization of laminin-5 (gamma2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy (2005) Biomaterials, 26, pp. 6280-6287; Buser, D., Weber, H.P., Donath, K., Fiorellini, J.P., Paquette, D.W., Williams, R.C., Soft tissue reactions to nonsubmerged unloaded titanium implants in beagle dogs (1992) J. Periodontol, 63, pp. 225-235; Abrahamsson, I., Zitzmann, N.U., Berglundh, T., Wennerberg, A., Lindhe, J., Bone and soft tissue integration to titanium implants with different surface topography: An experimental study in the dog (2001) Int. J. Oral. Maxillofac. Implants, 16, pp. 323-332; Berglundh, T., Lindhe, J., Dimension of the peri-implant mucosa. Biological width revisited (1996) J. Clin. Periodontol, 23, pp. 971-973; Chehroudi, B., Gould, T.R., Brunette, D.M., The role of connective tissue in inhibiting epithelial downgrowth on titanium-coated percutaneous implants (1992) J. Biomed. Mater. Res., 26, pp. 493-515; Fujiseki, M., Matsuzaka, K., Yoshinari, M., Shimono, M., Inoue, T., An experimental study on the features of periimplant epithelium: Immunohistochemical and electron-microscopic observations (2003) Bull. Tokyo Dent. Coll., 44, pp. 185-199; Hermann, J.S., Buser, D., Schenk, R.K., Schoolfield, J.D., Cochran, D.L., Biologic width around one-and twopiece titanium implants (2001) Clin. Oral. Implants Res., 12, pp. 559-571; Ikeda, H., Shiraiwa, M., Yamaza, T., Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri-implant or junctional epithelium of rat gingivae (2002) Clin. Oral. Implants Res., 13, pp. 243-251; Kawahara, H., Kawahara, D., Mimura, Y., Takashima, Y., Ong, J.L., Morphologic studies on the biologic seal of titanium dental implants. Report II. In vivo study on the defending mechanism of epithelial adhesions/attachment against invasive factors (1998) Int. J. Oral. Maxillofac. Implants, 13, pp. 465-473; Moon, I.S., Berglundh, T., Abrahamsson, I., Linder, E., Lindhe, J., The barrier between the keratinized mucosa and the dental implant. An experimental study in the dog (1999) J. Clin. Periodontol, 26, pp. 658-663; Rosengren, A., Johansson, B.R., Danielsen, N., Thomsen, P., Ericson, L.E., Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants (1996) Biomaterials, 17, pp. 1779-1786; Baschong, W., Suetterlin, R., Hefti, A., Schiel, H., Confocal laser scanning microscopy and scanning electron microscopy of tissue Ti-implant interfaces (2001) Micron, 32, pp. 33-41; Schupbach, P., Glauser, R., The defence architecture of the human peri-implant mucosa: A histological study (2007) J. Prosthet Dent., 97, pp. S15-S25; Dean III, J.W., Culbertson, K.C., D'Angelo, A.M., Fibronectin and laminin enhance gingival cell attachment to dental implant surfaces in vitro (1995) Int. J. Oral. Maxillofac. Implants, 10, pp. 721-728; Jansen, J.A., De Wijn, J.R., Wolters-Lutgerhorst, J.M., Van Mullem, P.J., Ultrastructural study of epithelial cell attachment to implant materials (1985) J. Dent. Res., 64, pp. 891-896; Kawahara, H., Kawahara, D., Hashimoto, K., Takashima, Y., Ong, J.L., Morphologic studies on the biologic seal of titanium dental implants. Report I. In vitro study on the epithelialization mechanism around the dental implant (1998) Int. J. Oral. Maxillofac. Implants, 13, pp. 457-464; Oates, T.W., Maller, S.C., West, J., Steffensen, B., Human gingival fibroblast integrin subunit expression on titanium implant surfaces (2005) J. Periodontol, 76, pp. 1743-1750; Pendegrass, C.J., Gordon, D., Middleton, C.A., Sun, S.N., Blunn, G.W., Sealing the skin barrier around transcutaneous implants: In vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy (2008) J. Bone Joint Surg. Br., 90, pp. 114-121; Säuberlich, S., Klee, D., Richter, E.J., Höcker, H., Spiekermann, H., Cell culture tests for assessing the tolerance of soft tissue to variously modified titanium surfaces (1999) Clin. Oral. Implants Res., 10, pp. 379-393; Schierano, G., Bellone, G., Manzella, C., Preti, G., Emanuelli, G., In vitro effect of transforming growth factor-beta on adhesion molecule expression by human gingival fibroblasts cultured in the presence of a titanium abutment (2001) J. Periodontol, 72, pp. 1658-1665; Radyuk, S.N., Mericko, P.A., Popova, T.G., Grene, E., Alibek, K., In vitro-generated respiratory mucosa: A new tool to study inhalational anthrax (2003) Biochem. Biophys. Res. Commun., 305, pp. 624-632; Locke, M., Hyland, P.L., Irwin, C.R., Mackenzie, I.C., Modulation of gingival epithelial phenotypes by interactions with regionally defined populations of fibroblasts (2008) J. Periodontal Res., 43, pp. 279-289; Moharamzadeh, K., Brook, I.M., Scutt, A.M., Thornhill, M.H., Van Noort, R., Mucotoxicity of dental composite resins on a tissue-engineered human oral mucosal model (2008) J. Dent., 36, pp. 331-336; Moharamzadeh, K., Brook, I.M., Van Noort, R., Scutt, A.M., Smith, K.G., Thornhill, M.H., Development, optimization and characterization of a full-thickness tissue engineered human oral mucosal model for biological assessment of dental biomaterials (2008) J. Mater. Sci. Mater. Med., 19, pp. 1793-1801; Moharamzadeh, K., Brook, I.M., Van Noort, R., Scutt, A.M., Thornhill, M.H., Tissue-engineered oral mucosa: A review of the scientific literature (2007) J. Dent. Res., 86, pp. 115-124; Moharamzadeh, K., Franklin, K.L., Brook, I.M., Van Noort, R., Biologic assessment of antiseptic mouthwashes using a three-dimensional human oral mucosal model (2009) J. Periodontol, 80, pp. 769-775; Richardson, K.C., Jarett, L., Finke, E.H., Embedding in epoxy resins for ultrathin sectioning in electron microscopy (1960) Stain Technol., 35, pp. 313-323; Bjursten, L.M., Emanuelsson, L., Ericson, L.E., Method for ultrastructural studies of the intact tissuemetal interface (1990) Biomaterials, 11, pp. 596-601; Vannet, B.V., Mohebbian, N., Wehrbein, H., Toxicity of used orthodontic archwires assessed by three-dimensional cell culture (2006) Eur. J. Orthod., 28, pp. 426-432; Vannet, B.M.V., Hanssens, J.L., Cytotoxicity of two bonding adhesives assessed by three-dimensional cell culture (2007) Angle Orthod., 77, pp. 716-722; Dongari-Bagtzoglou, A., Kashleva, H., Development of a novel three-dimensional in vitro model of oral Candida infection (2006) Microb. Pathog, 40, pp. 271-278; Wille, J.J., Mansson-Rahemtulla, B., Rahemtulla, F., Characterization of human gingival keratinocytes cultured in a serum-free medium (1990) Arch. Oral. Biol., 35, pp. 967-976; Mckinney Jr., R.V., Steflik, D.E., Koth, D.L., The biologic response to the single-crystal sapphire endosteal dental implant: Scanning electron microscopic observations (1984) J. Prosthet Dent., 51, pp. 372-379
Uncontrolled Keywords: Cell Cultured techniques/methods Dental implants Epithelial attachment Immunohistochemistry Mouth mucosa/anatomy &histology Tissue engineering biomedical and dental materials cytokeratin 10 cytokeratin 13 tissue scaffold titanium adult article audiovisual equipment cell adhesion coculture culture technique cytology dental etching epithelium cell female fibroblast histology human keratinocyte mouth mucosa scanning electron microscopy skin surface property tooth brushing tooth implantation tooth prosthesis Cell Culture Techniques Coculture Techniques Dental Materials Dental Polishing Dental Prosthesis Design Epithelial Cells Fibroblasts Histocytological Preparation Techniques Humans Keratin-10 Keratin-13 Keratinocytes Microscopy, Electron, Scanning Models, Anatomic Surface Properties Tissue Scaffolds Young Adult
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dept of General Dental Practice And Oral & Maxillofacial Imaging
Depositing User: Associate Professor Dr. Wen Lin Chai
Date Deposited: 08 May 2012 03:56
Last Modified: 11 Dec 2013 03:56

Actions (login required)

View Item View Item