Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor-the evidence of potent in vitro biological activities

Klaus, Anita and Wan-Mohtar, Wan Abd Al Qadr Imad and Nikolic, Biljana and Cvetkovic, Stefana and Vunduk, Jovana (2021) Pink oyster mushroom Pleurotus flabellatus mycelium produced by an airlift bioreactor-the evidence of potent in vitro biological activities. World Journal of Microbiology & Biotechnology, 37 (1). ISSN 0959-3993, DOI

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Four types of mycelial extracts were derived from the airlift liquid fermentation (ALF) of Pleurotus flabellatus, namely exopolysaccharide (EX), endopolysaccharide (EN), hot water (WE), and hot alkali (AE) extracts. Such extracts were screened for their active components and biological potential. EN proved to be most effective in inhibition of lipid peroxidation (EC50=1.710.02 mg/mL) and in Cupric ion reducing antioxidant capacity (CUPRAC) assay (EC50=2.910.01 mg TE/g). AE exhibited most pronounced ability to chelate ferrous ions (EC50=4.96 +/- 0.08 mg/mL) and to scavenge ABTS radicals (EC50=3.36 +/- 0.03 mg TE/g). beta -glucans and total phenols contributed most to the chelating ability and quenching of ABTS radicals. Inhibition of lipid peroxidation correlated best with total glucans, total proteins, and beta -glucans. Total proteins contributed most to CUPRAC antioxidant capacity. Antifungal effect was determined against Candida albicans ATCC 10231 (MIC: 0.019-0.625 mg/mL; MFC: 0.039-2.5 mg/mL), and towards C. albicans clinical isolate (MIC and MFC: 10.0-20.0 mg/mL). Comparison of cytotoxicity against colorectal carcinoma HCT 116 cells (IC50: 1.8 +/- 0.3-24.6 +/- 4.2 mg/mL) and normal lung MRC-5 fibroblasts (IC50: 17.0 +/- 4.2-42.1 +/- 6.1 mg/mL) showed that EN, and especially AE possess selective anticancer activity (SI values 3.41 and 9.44, respectively). Slight genotoxicity was observed only for AE and EX, indicating the low risk concerning this feature. Notable antioxidative and anticandidal activities, selective cytotoxicity against colorectal carcinoma cells, and absence/low genotoxicity pointed out that ALF-cultivated P. flabellatus mycelium could be considered as a valuable source of bioactive substances. Graphic abstract

Item Type: Article
Funders: Ministry of Education, Science and Technological Development of the Republic of Serbia (451-03-68/2020-14/200116)
Uncontrolled Keywords: Antioxidative; Antifungal; Cytotoxicity; Genotoxicity; Pleurotus flabellatus
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms Zaharah Ramly
Date Deposited: 19 Jul 2022 07:45
Last Modified: 19 Jul 2022 07:45

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