Bromophenol Blue Binding to Mammalian Albumins and Displacement of Albumin-Bound Bilirubin

Tayyab, Saad and Boh, Boon Kim and Kadir, Habsah Abdul (2008) Bromophenol Blue Binding to Mammalian Albumins and Displacement of Albumin-Bound Bilirubin. Pakistan Journal of Biological Sciences, 11 (20). pp. 2418-2422. ISSN 1028-8880, DOI

Full text not available from this repository.
Official URL:


Interaction of bromophenol blue (BPB) with serum albumins from different mammalian species, namely, human (HSA), bovine (BSA), goat (GSA), sheep (SSA), rabbit (RbSA), porcine (PSA) and dog (DSA) was studied using absorption and absorption difference spectroscopy. BPB-albumin complexes showed significant differences in the spectral characteristics, i.e., extent of bathochromic shift and hypochromism relative to the spectral features of free BPB. Absorption difference spectra of these complexes also showed variations in the position of maxima and absorption difference (ΔAbs.) values. Absorption difference spectra of different bilirubin (BR)-albumin complexes showed a significant blue shift accompanied by decrease in ΔAbs. values in presence of BPB which were indicative of the displacement of bound BR from its binding site in BR-albumin complexes. These changes in the difference spectral characteristics of BR-albumin complexes were more marked at higher BPB concentration. However, the extent of these changes was different for different BR-albumin complexes. Taken together, all these results suggest that BPB partially shares BR binding site on albumin and different mammalian albumins show differences in the microenvironment of the BR/BPB binding site. © 2008 Asian Network for Scientific Information.

Item Type: Article
Uncontrolled Keywords: Bilirubin displacement; Bromophenol blue; Ligand binding; Mammalian species; Serum albumin
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 01 Sep 2020 01:59
Last Modified: 01 Sep 2020 01:59

Actions (login required)

View Item View Item