Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens

Priyadarshini, S. and Mainal, Azizah and Sonsudin, Faridah and Yahya, Rosiyah and Alyousef, Abdullah A. and Mohammed, Arshad (2020) Biosynthesis of TiO2 nanoparticles and their superior antibacterial effect against human nosocomial bacterial pathogens. Research on Chemical Intermediates, 46 (2). pp. 1077-1089. ISSN 0922-6168, DOI

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Antibacterial titanium dioxide nanoparticles (TiO2NPs) were biologically synthesized by microwave irradiation of Cissus quadrangularis extract followed by calcination at 450 °C for 3 h. As-synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction analysis, and high-resolution transmission electron microscopy. The bioactivity of TiO2NPs against human nosocomial Gram-positive and Gram-negative bacterial pathogens (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) in liquid media was analyzed in the dark and under ultraviolet (UV, 365 nm) illumination. TiO2NPs demonstrated inhibitory effects against bacteria in dark, and enhanced bactericidal effect under UV illumination. Photoactivation generated holes (h+) and electrons (e−) on the surface of the TiO2NPs, inducing hydroxyl (OH−) and superoxide free radicals (.O2 −) reactive oxygen species (ROS) on the bacterial surface. Furthermore, the interaction between the pathogenic bacteria and TiO2NPs was investigated by bacterial killing assay, colony counting, and viability assay using confocal microscopy. The experimental results revealed that the photoenhanced antibacterial effect of TiO2NPs makes them worth considering as a disinfectant for hospital garments. © 2019, Springer Nature B.V.

Item Type: Article
Funders: Deanship of Scientific Research at King Saud University for funding this work through research group no. RG-1440-053
Uncontrolled Keywords: Anatase; Antibacterial; Nosocomial pathogens; Photocatalysis; Reactive oxygen species; TiO2 nanoparticles
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Centre for Foundation Studies in Science > Chemistry Division
Faculty of Science > Department of Chemistry
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 17 Aug 2020 04:18
Last Modified: 17 Aug 2020 04:18

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