Mixed-ligand copper(II) complex of quercetin regulate osteogenesis and angiogenesis

Vimalraj, Selvaraj and Rajalakshmi, Subramaniyam and Raj Preeth, Desingh and Vinoth Kumar, Sivasubramanian and Deepak, Thirumalai and Gopinath, Venkatraman and Murugan, Kadarkarai and Chatterjee, Suvro (2018) Mixed-ligand copper(II) complex of quercetin regulate osteogenesis and angiogenesis. Materials Science and Engineering: C, 83. pp. 187-194. ISSN 0928-4931, DOI https://doi.org/10.1016/j.msec.2017.09.005.

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Official URL: https://doi.org/10.1016/j.msec.2017.09.005


Copper(II) complex of quercetin Cu + Q, mixed ligand complexes, quercetin-Cu(II)-phenanthroline [Cu + Q(PHt)] and quercetin-Cu(II)-neocuproine [Cu + Q(Neo)] have been synthesized and characterized. From the FT-IR spectroscopic studies, it was evident that C-ring of quercetin is involved in the metal chelation in all the three copper complexes. C-ring chelation was further proven by UV–Visible spectra and the presence of Cu(II) from EPR spectroscopic investigations. These complexes were found to have osteogenic and angiogenic properties, observed through in vitro osteoblast differentiation and chick embryo angiogenesis assay. In osteoblast differentiation, quercetin-Cu(II) complexes treatment increased calcium deposition and alkaline phosphatase activity (ALP) activity at the cellular level and stimulated Runx2 mRNA and protein, ALP mRNA and type 1 collagen mRNA expression at the molecular level. Among the complexes, Q + Cu(PHt) showed more effects on osteoblast differentiation when compared to that of other two copper complexes. Additionally, Q + Cu(Neo) showed more effect compared to Q + Cu. Furthermore, the effect of these complexes on osteoblast differentiation was confirmed by the expression of osteoblast specific microRNA, pre-mir-15b. The chick embryo angiogenesis assay showed that angiogenic parameters such as blood vessel length, size and junctions were stimulated by these complexes. Thus, the present study demonstrated that quercetin copper(II) complexes exhibit as a pharmacological agent for the orthopedic application.

Item Type: Article
Funders: Department of Science and Technology, Science and Engineering Research Board (SERB), India: (grant no. PDF/2015/000133), DST-Inspire Faculty (DST/INSPIRE/04/2015/001945) program, University Grant Commission-Faculty Research Program (UGC-FRP), Government of India
Uncontrolled Keywords: Quercetin; Cu(II) complexes; Osteoblast differentiation; Angiogenesis; microRNA
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 21 Feb 2019 03:47
Last Modified: 21 Feb 2019 03:47
URI: http://eprints.um.edu.my/id/eprint/20421

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