Cytokine expression profile of dengue patients at different phases of illness

Rathakrishnan, A. and Wang, S.M. and Hu, Y.L. and Khan, A.M. and Ponnampalavanar, S. and Lum, L.C.S. and Manikam, R. and Sekaran, S.D. (2012) Cytokine expression profile of dengue patients at different phases of illness. PLoS ONE, 7 (12). ISSN 1932-6203,

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Background: Dengue is an important medical problem, with symptoms ranging from mild dengue fever to severe forms of the disease, where vascular leakage leads to hypovolemic shock. Cytokines have been implicated to play a role in the progression of severe dengue disease; however, their profile in dengue patients and the synergy that leads to continued plasma leakage is not clearly understood. Herein, we investigated the cytokine kinetics and profiles of dengue patients at different phases of illness to further understand the role of cytokines in dengue disease. Methods and Findings: Circulating levels of 29 different types of cytokines were assessed by bead-based ELISA method in dengue patients at the 3 different phases of illness. The association between significant changes in the levels of cytokines and clinical parameters were analyzed. At the febrile phase, IP-10 was significant in dengue patients with and without warning signs. However, MIP-1 beta was found to be significant in only patients with warning signs at this phase. IP-10 was also significant in both with and without warning signs patients during defervescence. At this phase, MIP-1b beta and G-CSF were significant in patients without warning signs, whereas MCP-1 was noted to be elevated significantly in patients with warning signs. Significant correlations between the levels of VEGF, RANTES, IL-7, IL-12, PDGF and IL-5 with platelets; VEGF with lymphocytes and neutrophils; G-CSF and IP-10 with atypical lymphocytes and various other cytokines with the liver enzymes were observed in this study. Conclusions: The cytokine profile patterns discovered between the different phases of illness indicate an essential role in dengue pathogenesis and with further studies may serve as predictive markers for progression to dengue with warning signs.

Item Type: Article
Additional Information: ISI Document Delivery No.: 060RA Times Cited: 8 Cited Reference Count: 64 Rathakrishnan, Anusyah Wang, Seok Mui Hu, Yongli Khan, Asif M. Ponnampalavanar, Sasheela Lum, Lucy Chai See Manikam, Rishya Sekaran, Shamala Devi High Impact Research (HIR) J-00000-73560; University Malaya Research Grant (UMRG) RG081/09HTM; University Malaya Peruntukan Jangka Pendek (UMPJP) FS245/008B Project was funded by High Impact Research (HIR) J-00000-73560; University Malaya Research Grant (UMRG) RG081/09HTM; and University Malaya Peruntukan Jangka Pendek (UMPJP) FS245/008B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Public library science San francisco
Uncontrolled Keywords: Monocyte chemoattractant protein-1 platelet-activating-factor necrosis-factor-alpha hemorrhagic-fever virus-infection endothelial-cells interferon-gamma disease severity liver-disease inducible protein-10
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 18 Jun 2014 00:57
Last Modified: 05 Jul 2017 01:31

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