Effect of carbenicillin on somatic embryos formation of papaya (Carica papaya L. var. Eksotika I)

Fhaizal, M. and Vilasini, P. and Abd Rahman, Noorsaadah and Khalid, Norzulaani (2006) Effect of carbenicillin on somatic embryos formation of papaya (Carica papaya L. var. Eksotika I). Malaysian Journal of Science, 25 (2). pp. 47-54. ISSN 1394-3065,

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Abstract

Embryogenic callus could be established from immature embryo of Carica papaya L. var. Eksotika I after 3 - 4 months of culture on Callus Induction (CI) medium. Embryogenic callus was successfully induced (100 ) on CI medium supplemented with 250 mg/L Carbenicillin plus 10 mg/L 2,4-dichlorophenoxyacetic acids (2,4-D). Transparent somatic embryos then proliferated from the light brown friable embryogenic callus. Carbenicillin that was frequently used as an antibiotic to prevent fungal contamination in in-vitro cultures was found to stimulate the growth of somatic embryos of Carica papaya L. var. Eksotika I. Somatic embryos were maintained in a medium containing either reduced or without 2,4-D. The cultures could be maintained for a period of 5 - 6 months with no apparent loss of regenerative potential. The somatic embryos germinated on Germination (G) medium supplemented with 0.2 mg/L 6-Benzylaminopurine (BAP) and 2.0 mg/L 1-Napthaleneacetic acids (NAA) produced high regeneration frequency (88.41 ) with initial development of hypocotyls followed by rapid growth of plantlets. The in vitro shoots were readily rooted in G medium supplemented with 0.5 mg/L Indole-3-butyric acid (IBA) with 75 successful rate.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Somatic embryogenesis, Carica papaya, carbenicillin
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 29 Apr 2013 01:47
Last Modified: 28 Apr 2021 06:47
URI: http://eprints.um.edu.my/id/eprint/5936

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