Pang, Jun-Rui and How, Sher-Wei and Wong, Kah-Hui and Lim, Siew-Huah and Phang, Siew-Moi and Yow, Yoon-Yen (2022) Cholinesterase inhibitory activities of neuroprotective fraction derived from red alga Gracilaria manilaensis. Fisheries and Aquatic Sciences, 25 (2). 49 -63. ISSN 2234-1757,
Full text not available from this repository.Abstract
Anti-cholinesterase (ChE)s are commonly prescribed as the symptomatic treatment of Alzheimer’s disease. They are applied to prevent the breakdown of neurotransmitter acetylcholine (ACh) that bind to muscarinic and nicotinic receptors in the synaptic cleft. Seaweeds are one of the richest sources of bioactive compounds for both nutraceuticals and pharmacognosy applications. This study aimed to determine the anti-ChEs activity of Gracilaria manilaensis, one of the red seaweeds notables for its economic importance as food and raw materials for agar production. Methanol extracts (GMM) of G. manilaensis were prepared through maceration, and further purified with column chromatography into a semi-pure fraction. Ellman assay was carried out to determine the anti-acetylcholinesterase (AChE) and anti-butyrylcholinesterase (BuChE) activities of extracts and fractions. Lineweav-er-Burk plot analysis was carried out to determine the inhibition kinetic of potent extract and fraction. Major compound(s) from the most potent fraction was determined by liquid chromatography-mass spectrometry (LCMS). GMM and fraction G (GMMG) showed significant inhibitory activity AChE with EC50 of 2.6 mg/mL and 2.3 mg/mL respectively. GMM and GMMG exhibit mixed-inhibition and uncompetitive inhibition respectively against AChE. GMMG possesses neuroprotective compounds such as cynerine A, graveolinine, militarinone A, eplerenone and curumenol. These findings showed a promising insight of G. mani-laensis to be served as a nutraceutical for neuronal health care in the future. © 2022 The Korean Society of Fisheries and Aquatic Science.
| Item Type: | Article |
|---|---|
| Funders: | None |
| Uncontrolled Keywords: | Gracilaria manilaensis; Alzheimer’s disease; Graveolinine; Mixed-inhibition; Uncompetitive inhibition; Cholinesterases |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Faculty of Medicine Faculty of Science > Department of Chemistry |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 16 Nov 2023 06:49 |
| Last Modified: | 16 Nov 2023 06:49 |
| URI: | http://eprints.um.edu.my/id/eprint/43341 |
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