Al-Masawa, Maimonah-Eissa and Wan Kamarul Zaman, Wan Safwani and Chua, Kien-Hui (2020) Biosafety evaluation of culture-expanded human chondrocytes with growth factor cocktail: a preclinical study. Scientific Reports, 10 (1). ISSN 2045-2322,
Full text not available from this repository.Abstract
The scarcity of chondrocytes is a major challenge for cartilage tissue engineering. Monolayer expansion is necessary to amplify the limited number of chondrocytes needed for clinical application. Growth factors are often added to improve monolayer culture conditions, promoting proliferation, and enhancing chondrogenesis. Limited knowledge on the biosafety of the cell products manipulated with growth factors in culture has driven this study to evaluate the impact of growth factor cocktail supplements in chondrocyte culture medium on chondrocyte genetic stability and tumorigenicity. The growth factors were basic fibroblast growth factor (b-FGF), transforming growth factor beta 2 (TGF beta 2), insulin-like growth factor 1 (IGF-1), insulin-transferrin-selenium (ITS), and platelet-derived growth factor (PD-GF). Nasal septal chondrocytes cultured in growth factor cocktail exhibited a significantly high proliferative capacity. Comet assay revealed no significant DNA damage. Flow cytometry showed chondrocytes were mostly at G0-G1 phase, exhibiting normal cell cycle profile with no aneuploidy. We observed a decreased tumour suppressor genes' expression (p53, p21, pRB) and no TP53 mutations or tumour formation after 6 months of implantation in nude mice. Our data suggest growth factor cocktail has a low risk of inducing genotoxic and tumorigenic effects on chondrocytes up to passage 6 with 16.6 population doublings. This preclinical tumorigenicity and genetic instability evaluation is crucial for further clinical works.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Animals; Cell Cycle; Cell Proliferation; Cell Shape; Chondrocytes; Chondrogenesis; Culture Media |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Engineering Faculty of Engineering > Biomedical Engineering Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 28 Nov 2023 01:08 |
| Last Modified: | 29 Nov 2023 04:14 |
| URI: | http://eprints.um.edu.my/id/eprint/36187 |
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