Liew, Kok Jun and Lim, Lily and Woo, Hui Ying and Chan, Kok Gan and Shamsir, Mohd Shahir and Goh, Kian Mau (2018) Purification and characterization of a novel GH1 beta-glucosidase from Jeotgalibacillus malaysiensis. International Journal of Biological Macromolecules, 115. pp. 1094-1102. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2018.04.156.
Full text not available from this repository.Abstract
Beta-glucosidase (BGL) is an important industrial enzyme for food, waste and biofuel processing. Jeotgalibacillus is an understudied halophilic genus, and no beta-glucosidase from this genus has been reported. A novel beta-glucosidase gene (1344 bp) from J. malaysiensis DSM 28777T was cloned and expressed in E. coli. The recombinant protein, referred to as BglD5, consists of a total 447 amino acids. BglD5 purified using a Ni-NTA column has an apparent molecular mass of 52 kDa. It achieved the highest activity at pH 7 and 65 °C. The activity and stability were increased when CaCl2 was supplemented to the enzyme. The enzyme efficiently hydrolyzed salicin and (1 → 4)-beta-glycosidic linkages such as in cellobiose, cellotriose, cellotetraose, cellopentose, and cellohexanose. Similar to many BGLs, BglD5 was not active towards polysaccharides such as Avicel, carboxymethyl cellulose, Sigmacell cellulose 101, alpha-cellulose and xylan. When BglD5 blended with Cellic® Ctec2, the total sugars saccharified from oil palm empty fruit bunches (OPEFB) was enhanced by 4.5%. Based on sequence signatures and tree analyses, BglD5 belongs to the Glycoside Hydrolase family 1. This enzyme is a novel beta-glucosidase attributable to its relatively low sequence similarity with currently known beta-glucosidases, where the closest characterized enzyme is the DT-Bgl from Anoxybacillus sp. DT3-1.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Teknologi Malaysia Research Grants (Grant No. 16H89, & 14H67), PPP (GA001-2016, GA002-2016, PG226-2016), Zamalah Program of Universiti Teknologi Malaysia |
| Uncontrolled Keywords: | beta-Glucosidase; Biotechnology; Cellobiose; Cloning, Molecular; Computational Biology; Kinetics; Molecular Weight; Planococcaceae; Substrate Specificity |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Jun 2019 04:26 |
| Last Modified: | 17 Jun 2019 04:26 |
| URI: | http://eprints.um.edu.my/id/eprint/21471 |
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