Lectin-functionalized carboxymethylated kappa-carrageenan microparticles for oral insulin delivery

Leong, Kok Hoong (2011) Lectin-functionalized carboxymethylated kappa-carrageenan microparticles for oral insulin delivery. Carbohydrate Polymers, 86 (2). pp. 555-565. DOI https://doi.org/10.1016/j.carbpol.2011.04.070.

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Abstract

We hypothesized that pH-responsive carboxymethylated kappa-carrageenan microparticles could protect entrapped oral insulin from acidic and proteolytic degradation in the gastrointestinal tract. Therefore, the objectives of this study were to prepare and characterize insulin entrapped in lectin-functionalized carboxymethylated kappa-carrageenan microparticles and to evaluate their therapeutic efficacy in vitro and in vivo. The encapsulation of insulin was performed using an ionic gelation technique and was optimized to give an encapsulation efficiency of 94.2 ± 2.6% and a drug-loading capacity of 13.5 ± 0.4%. The microparticles were further surface-lectin-functionalized for improved intestinal mucoadhesiveness. The oral administration of insulin entrapped in the microparticles led to a prolonged duration of the hypoglycemic effect, up to 12–24 h, in diabetic rats. From the release profile and the low toxicity of the microparticles, it can be concluded that these lectin-functionalized carboxymethylated kappa-carrageenan microparticles have the potential to be developed into an oral insulin delivery system.

Item Type:	Article
Funders:	University of Malaya, Malaysia (Grant No: FS328/2008C), Tacara Sdn. Bhd
Uncontrolled Keywords:	Carrageenan; Insulin; Lectin; Microparticles; Oral delivery
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Medicine
Depositing User:	Dr. Leong Kok Hoong
Date Deposited:	22 May 2014 02:22
Last Modified:	22 May 2014 02:22
URI:	http://eprints.um.edu.my/id/eprint/9999

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