Nair, S. and Thong, Kwai Lin and Pang, T. and Altwegg, M. (2002) PCR-Single strand conformation polymorphism (SSCP) analysis of Salmonella typhi and other Salmonella serovars. Journal of Clinical Microbiology, 40 (7). pp. 2346-2351.
![[img]](http://eprints.um.edu.my/style/images/fileicons/application_pdf.png)
|
PDF (Nair et al., 2002)
Nair_et_al.,_2002.pdf - Published Version Download (1MB) |
Abstract
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR¨Csingle-strand conformation polymorphism(PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Prof. Dr Kwai Lin Thong |
| Date Deposited: | 12 Nov 2014 04:14 |
| Last Modified: | 12 Oct 2018 06:33 |
| URI: | http://eprints.um.edu.my/id/eprint/9966 |
Actions (login required)
 |
View Item |
