Technical improvement in DNA preparation in pulsed-field gel electrophoresis

Thong, Kwai Lin and Lai, K.S. and Goh, Yee Ling (2003) Technical improvement in DNA preparation in pulsed-field gel electrophoresis. Malaysian Journal of Science, 22 (2). pp. 55-59. ISSN 1394-3065

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Abstract

We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 12 Nov 2014 03:30
Last Modified: 27 Apr 2021 07:58
URI: http://eprints.um.edu.my/id/eprint/9949

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