Technical improvement in DNA preparation in pulsed-field gel electrophoresis

Thong, K.L.; Lai, K.S.; Goh, Yee-Ling (2003) Technical improvement in DNA preparation in pulsed-field gel electrophoresis. Malaysian Journal of Science, 22 (2). pp. 55-59.

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    Abstract

    We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible.

    Item Type: Article
    Creators:
    1. Thong, K.L.(Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Lai, K.S.
    3. Goh, Yee-Ling
    Journal or Publication Title: Malaysian Journal of Science
    Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Subjects: Q Science > QR Microbiology
    Divisions: Faculty of Science > Institute of Biological Sciences
    Depositing User: Prof. Dr Kwai Lin Thong
    Date Deposited: 12 Nov 2014 11:30
    Last Modified: 14 Dec 2017 12:10
    URI: http://eprints.um.edu.my/id/eprint/9949

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