Growth inhibitory response and ultrastructural modification of oral-associated candidal reference strains (ATCC) by Piper betle L. extract

Nordin, M.A.F. and Himratul-Aznita, Wan Harun and Razak, Fathilah Abdul and Musa, M.Y. (2014) Growth inhibitory response and ultrastructural modification of oral-associated candidal reference strains (ATCC) by Piper betle L. extract. International Journal of Oral Science, 6. pp. 15-21. ISSN 2049-3169, DOI https://doi.org/10.1038/ijos.2013.97.

Preview

PDF (Open Access Journal) ijos201397a.pdf Download (3MB)

Abstract

Candida species have been associated with the emergence of strains resistant to selected antifungal agents. Plant products have been used traditionally as alternative medicine to ease mucosal fungal infections. This study aimed to investigate the effects of Piper betle extract on the growth profile and the ultrastructure of commonly isolated oral candidal cells. The major component of P. betle was identified using liquid chromatography-mass spectrophotometry (LC-MS/MS). Seven ATCC control strains of Candida species were cultured in yeast peptone dextrose broth under four different growth environments: (i) in the absence of P. betle extract; and in the presence of P. betle extract at respective concentrations of (ii) 1 mg⋅mL−1; (iii) 3 mg⋅mL−1; and (iv) 6 mg⋅mL−1. The growth inhibitory responses of the candidal cells were determined based on changes in the specific growth rates (µ). Scanning electron microscopy (SEM) was used to observe any ultrastructural alterations in the candida colonies. LC-MS/MS was performed to validate the presence of bioactive compounds in the extract. Following treatment, it was observed that the µ-values of the treated cells were significantly different than those of the untreated cells (P<0.05), indicating the fungistatic properties of the P. betle extract. The candidal population was also reduced from an average of 13.44×106 to 1.78×106 viable cell counts (CFU)⋅mL−1. SEM examination exhibited physical damage and considerable morphological alterations of the treated cells. The compound profile from LC-MS/MS indicated the presence of hydroxybenzoic acid, chavibetol and hydroxychavicol in P. betle extract. The effects of P. betle on candida cells could potentiate its antifungal activity.

Item Type:	Article
Funders:	UNSPECIFIED
Uncontrolled Keywords:	Antifungal activity; Candida; Cell morphology; Growth inhibitory effect; Piper betle L.
Subjects:	R Medicine > RK Dentistry
Divisions:	Faculty of Dentistry
Depositing User:	Mr Ahmad Azwan Azman
Date Deposited:	02 May 2014 01:16
Last Modified:	23 Dec 2019 07:45
URI:	http://eprints.um.edu.my/id/eprint/9876

Actions (login required)

View Item