Gastroprotective mechanism of Bauhinia thonningii Schum

Abdelwahab, S.I. and Taha, M.M.E. and Abdulla, M.A. and Nordin, N. and Hadi, A.H.A. and Mohan, S. and Jayapalan, J.J. and Hashim, Onn Haji (2013) Gastroprotective mechanism of Bauhinia thonningii Schum. Journal of Ethnopharmacology, 148 (1). pp. 277-286. ISSN 0378-8741, DOI https://doi.org/10.1016/j.jep.2013.04.027.

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Abstract

Ethnopharmacological relevance: Bauhinia thonningii Schum. (Cesalpiniaceae) is locally known as Tambarib and used to treat various diseases including gastric ulcer. Aim of the study: The current study aims to evaluate the gastroprotecive mechanism(s) of methanolic (MEBT) and chloroform (CEBT) extracts of Bauhinia thonningii leaves on ethanol-induced gastric ulceration. Materials and methods: Gastric acidity, quantification and histochemistry of mucus, gross and microscopic examination, nitric oxide, lipid peroxidation, 2D gel electrophoresis, mass spectroscopy and biochemical tests were utilized to assess the mechanism(s) underlying the gastroprotective effects of MEBT and CEBT. Effect of these extracts into lipopolysaccharide/interferon-gamma stimulated rodent cells were done in vitro. In vitro and in vivo toxicity studies were also conducted. Antioxidant activities of MEBT and CEBT were examined using DPPH, FRAP and ORAC assays. Phytochemical analyses of MEBT and CEBT were conducted using chemical and spectroscopic methods. Results: Gross and histological features confirmed the anti-ulcerogenic properties of Bauhinia thonningii. Gastroprotective mechanism of MEBT was observed to be mediated through the modulation of PAS-reactive substances, MDA and proteomics biomarkers (creatine kinase, malate dehydrogenase, ATP synthase, actin and thioredoxin). MEBT and CEBT showed no significant in vitro and in vivo effects on nitric oxide. Methanolic extract (MEBT) showed superior gastroprotective effects, polyphenolic content and antioxidant activities compared to CEBT. The plant extracts showed no in vitro or in vivo toxicity. Conclusion: It could be concluded that MEBT possesses anti-ulcer activity, which could be attributed to the inhibition of ethanol-induced oxidative damage and the intervention in proteomic pathways but not the nitric oxide pathway. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	ISI Document Delivery No.: 174NP Times Cited: 1 Cited Reference Count: 33 Abdelwahab, Siddig Ibrahim Taha, Manal Mohamed Elhassan Abdulla, Mahmood Ameen Nordin, Norazie Hadi, A. Hamid A. Mohan, Syam Jayapalan, Jaime Jacqueline Hashim, Onn Haji University of Malaya HIR-UM-MOHE F00009-21001 The authors would like to express their utmost gratitude and appreciation to University of Malaya (HIR-UM-MOHE F00009-21001) for providing grant to conduct this study. Elsevier ireland ltd Clare
Uncontrolled Keywords:	Bauhinia thonningii Schum Gastric ulcer Antioxidation Nitric oxide Proteomic analysis antioxidant activity mass spectrometry stem bark growth assay extracts savanna climate plants fire
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	23 Apr 2014 03:25
Last Modified:	24 Oct 2019 08:28
URI:	http://eprints.um.edu.my/id/eprint/9847

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