Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design

Jamil, Z. and Mohamad Annuar, M.S. and Ibrahim, S. and Vikineswary, S. (2009) Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design. International Journal of Hydrogen Energy, 34 (17). pp. 7502-7512. ISSN 0360-3199, DOI https://doi.org/10.1016/j.ijhydene.2009.05.116.

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Abstract

Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100 (v/v) POME concentration, 10 (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71 respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85. © 2009 International Association for Hydrogen Energy.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	498TI Times Cited:18 Cited References Count:34
Uncontrolled Keywords:	Biohydrogen production, Photosynthetic bacteria, POME, Response surface methodology, Rhodopseudomonas palustris, Bacteria, Bacteriology, Gas producers, Light transmission, Optimization, Regression analysis, Sulfur, Surface analysis, Surface properties, Vegetable oils, Hydrogen production.
Subjects:	T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General)
Divisions:	Faculty of Engineering
Depositing User:	Mr Jenal S
Date Deposited:	26 Feb 2014 01:44
Last Modified:	03 Jul 2017 09:02
URI:	http://eprints.um.edu.my/id/eprint/9433

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