Production of itaconic acid from jatropha curcas seed cake by aspergillus terreus

Ahmed El-Imam, A.M. and Kazeem, M.O. and Odebisi, M.B. and Oke, M.A. and Abidoye, A.O. (2013) Production of itaconic acid from jatropha curcas seed cake by aspergillus terreus. Notulae Scientia Biologicae , 5 (1). pp. 57-61. ISSN 2067-3205

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Abstract

Submerged substrate fermentation of Jatropha seed cake, a by-product of oil extraction from Jatropha curcas seed was carried out using Aspergillus terreus for the production of itaconic acid. The Jatropha seed cake was initially converted into fermentable sugars by dilute acid hydrolysis using 50% sulphuric acid. The rate of hydrolysis was 1.04 gL-1. The fermentation process was carried out at room temperature, agitation of 400 rpm and three physico-chemical parameters (pH, inoculum size and substrate concentration) were varied. Itaconic acid and glucose assays were carried out by spectrophotometry and Dinitrosalicylic acid methods respectively daily. Maximum yield of itaconic acid was 48.70 gL-1 at 5 ml of inoculum size, 50% substrate concentration and pH 1.5. The residual glucose concentration increased for the first two days of fermentation after which it began to decrease as the itaconic acid concentration increased. The least concentration of itaconic acid observed was 6.00 gL-1, obtained after 24 hours of fermentation with 4 ml inoculum size, 50% substrate concentration and at pH 1.5. The findings of this work indicate that Jatropha curcasseed cake is a suitable substrate for itaconic acid production.

Item Type: Article
Uncontrolled Keywords: Acid hydrolysis, Aspergillus terreus, fermentation, itaconic acid, Jatropha seed cake, residual glucose
Subjects: Q Science > QR Microbiology
S Agriculture > S Agriculture (General)
T Technology > TP Chemical technology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Mushafau Adebayo Oke
Date Deposited: 12 Feb 2014 01:34
Last Modified: 12 Feb 2014 01:34
URI: http://eprints.um.edu.my/id/eprint/9254

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