Abdelwahab, Siddig Ibrahim and Mohan, Syam and Elhassan, Manal Mohamed and Al-Mekhlafi, Nabil and Mariod, Abdelbasit Adam and Abdul, Ahmad Bustamam and Abdulla, Mahmood Ameen and Alkharfy, Khalid M. (2011) Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine, 2011. p. 156765. ISSN 1741-427X, DOI 3018659.
Full text not available from this repository.Abstract
Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Orthosiphon stamineus; |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > School of Medicine |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 25 Feb 2011 03:56 |
| Last Modified: | 29 Jan 2020 06:56 |
| URI: | http://eprints.um.edu.my/id/eprint/89 |
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