Genetic assemblage of Sarcocystis spp. in Malaysian snakes

Lau, Y.L.; Chang, P.Y.; Subramaniam, V.; Ng, Y.H.; Mahmud, R.; Ahmad, A.F.; Fong, M.Y. (2013) Genetic assemblage of Sarcocystis spp. in Malaysian snakes. Parasites & Vectors, 6. p. 257. ISSN 1756-3305

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    Abstract

    Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods: In this study, fecal specimens of several snake species in Malaysia were examined for the presence of Sarcocystis by PCR of 18S rDNA sequence. Microscopy examination of the fecal specimens for sporocysts was not carried as it was difficult to determine the species of the infecting Sarcocystis. Results: Of the 28 snake fecal specimens, 7 were positive by PCR. BLASTn and phylogenetic analyses of the amplified 18S rDNA sequences revealed the snakes were infected with either S. nesbitti, S. singaporensis, S. zuoi or undefined Sarcocystis species. Conclusion: This study is the first to report Sarcocystis infection in a cobra, and S. nesbitti in a reticulated python.

    Item Type: Article
    Creators:
    1. Lau, Y.L.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Chang, P.Y.
    3. Subramaniam, V.
    4. Ng, Y.H.
    5. Mahmud, R.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    6. Ahmad, A.F.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    7. Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Parasites & Vectors
    Additional Information: Lau, Yee Ling Chang, Phooi Yee Subramaniam, Vellayan Ng, Yit Han Mahmud, Rohela Ahmad, Arine Fadzlun Fong, Mun Yik
    Uncontrolled Keywords: Definitive host, Sarcocystis, Snake, 18S rDNA, Phylogenetic analysis
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 16 Dec 2013 11:59
    Last Modified: 16 Dec 2013 11:59
    URI: http://eprints.um.edu.my/id/eprint/8686

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