Lee, S.K. and Tan, K.W. and Ng, S.W. and Ooi, K.K. and Ang, K.P. and Abdah, M.A. (2013) Zinc (II) complex with a cationic Schiff base ligand: Synthesis, characterization, and biological studies. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN 1386-1425, DOI PMID: 24231745.
Full text not available from this repository.Abstract
A cationic Schiff base ligand, TSB (L) and its Zn (II) complex (1) were synthesized and characterized by using CHN, 1H-NMR, FT-IR, UV, LC-MS, and X-ray methods. Their ability to inhibit topoisomerase I, DNA cleavage activities, and cytotoxicity were studied. X-ray diffraction study shows that the mononuclear complex 1 is four coordinated with distorted tetrahedral geometry. The singly deprotonated Schiff base ligand L acts as a bidentate ON-donor ligand. Complexation of L increases the inhibitory strength on topoisomerase I activity. Complex 1 could fully inhibit topoisomerase I activity at 250μM, while L did not show any inhibitory effect on topoisomerase I activity. In addition, L and complex 1 could cleave pBR322 DNA in a concentration and time dependent profile. Surprisingly, L has better DNA cleavage activity than complex 1. The cleavage of DNA by complex 1 is altered in the presence of hydrogen peroxide. Furthermore, L and complex 1 are mildly cytotoxic towards human ovarian cancer A2780 and hepatocellular carcinoma HepG2.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Chemistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. |
| Uncontrolled Keywords: | Cytotoxicity, DNA cleavage, Schiff base, Topoisomerase I, Zinc |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 21 Nov 2013 09:26 |
| Last Modified: | 21 Nov 2013 09:26 |
| URI: | http://eprints.um.edu.my/id/eprint/8562 |
Actions (login required)
 |
View Item |
