Alpha-tomatine synergises with paclitaxel to enhance apoptosis of androgen-independent human prostate cancer PC-3 cells in vitro and in vivo

Lee, Sui-Ting and Wong, Pooi Fong and Hooper, J.D. and Mustafa, Mohd Rais (2013) Alpha-tomatine synergises with paclitaxel to enhance apoptosis of androgen-independent human prostate cancer PC-3 cells in vitro and in vivo. Phytomedicine, 20 (14). pp. 1297-1305. ISSN 0944-7113, DOI https://doi.org/10.1016/j.phymed.2013.07.002.

[img] PDF
1-s2.0-S0944711313002468-main.pdf - Published Version
Restricted to Registered users only
Download (3MB) | Request a copy
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

Alpha (α)-tomatine, a major saponin found in tomato has been shown to inhibit the growth of androgen-independent prostate cancer PC-3 cells. The effects of α-tomatine in combination with the chemotherapeutic agent paclitaxel against PC-3 cells were investigated in the present study. Combined treatment with a sub-toxic dose of α-tomatine and paclitaxel significantly decreased cell viability with concomitant increase in the percentage of apoptotic PC-3 cells. The combined treatment, however, had no cytotoxic effect on the non-neoplastic prostate RWPE-1 cells. Apoptosis of PC-3 cells was accompanied by the inhibition of PI3K/Akt pro-survival signaling, an increase in the expression of the pro-apoptotic protein BAD but a decrease in the expressions of anti-apoptotic proteins, Bcl-2 and Bcl-xL. Results from a mouse xenograft model showed the combined treatment completely suppressed subcutaneous tumor growth without significant side effects. Consistent with its in vitro anti-cancer effects, tumor materials from mice showed increased apoptosis of tumor cells with reduced protein expression of activated PI3K/Akt. These results suggest that the synergistic anti-cancer effects of paclitaxel and α-tomatine may be beneficial for refractory prostate cancer treatment.

Item Type: Article
Funders: UNSPECIFIED
Uncontrolled Keywords: Alpha-tomatine; Paclitaxel; Prostate cancer; Apoptosis; PC-3 tumor xenograft
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms Haslinda Lahuddin
Date Deposited: 11 Nov 2013 03:35
Last Modified: 18 Dec 2019 06:44
URI: http://eprints.um.edu.my/id/eprint/8531

Actions (login required)

View Item View Item
UM Research Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.