Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity

Wada, A.; Wong, P.F.; Hojo, H.; Hasegawa, M.; Ichinose, A.; Llanes, R.; Kubo, Y.; Senba, M.; Ichinose, Y. (2013) Alarin but not its alternative-splicing form, GALP (Galanin-like peptide) has antimicrobial activity. Biochemical and Biophysical Research Communications, 434 (2). pp. 223-227. ISSN 0006-291X

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    Abstract

    Alarin is an alternative-splicing form of GALP (galanin-like peptide). It shares only 5 conserved amino acids at the N-terminal region with GALP which is involved in a diverse range of normal brain functions. This study seeks to investigate whether alarin has additional functions due to its differences from GALP. Here, we have shown using a radial diffusion assay that alarin but not GALP inhibited the growth of Escherichia coli (strain ML-35). The conserved N-terminal region, however, remained essential for the antimicrobial activity of alarin as truncated peptides showed reduced killing effect. Moreover, alarin inhibited the growth of E. coli in a similar potency as human cathelicidin LL-37, a well-studied antimicrobial peptide. Electron microscopy further showed that alarin induced bacterial membrane blebbing but unlike LL-37, it did not cause hemolysis of erythrocytes. In addition, alarin is only active against the gram-negative bacteria, E. coli but not the gram-positive bacteria, Staphylococcus aureus. Thus, these data suggest that alarin has potentials as an antimicrobial and should be considered for the development in human therapeutics. (c) 2013 Elsevier Inc. All rights reserved.

    Item Type: Article
    Creators:
    1. Wada, A.
    2. Wong, P.F.(Department of Pharmacology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    3. Hojo, H.
    4. Hasegawa, M.
    5. Ichinose, A.
    6. Llanes, R.
    7. Kubo, Y.
    8. Senba, M.
    9. Ichinose, Y.
    Journal or Publication Title: Biochemical and Biophysical Research Communications
    Additional Information: Wada, Akihiro Wong, Pooi-Fong Hojo, Hironobu Hasegawa, Makoto Ichinose, Akitoyo Llanes, Rafael Kubo, Yoshinao Senba, Masachika Ichinose, Yoshio
    Uncontrolled Keywords: Alarin; Galanin; LL-37; Antimicrobial peptide; Escherichia coli; Staphylococcus aureus; Hemolysis; Membrane blebbing
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 11 Nov 2013 11:38
    Last Modified: 11 Nov 2013 11:38
    URI: http://eprints.um.edu.my/id/eprint/8530

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