Development of species level DNA barcodes to identify cultivated edible mushrooms

Avin, F.A. and Vikineswary, S. and Shin, T.Y. and Bhassu, S. (2012) Development of species level DNA barcodes to identify cultivated edible mushrooms. In: The 18th Congress of the International Society for Mushroom Science, 26-30 Aug 2012, Beijing, China.

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Abstract

The majority of edible mushrooms belong to Basidiomycetes and these mushrooms have become more important in recent times for their medicinal and nutritional properties. For many years, species of this phylum have been mainly classified by their phenotypic traits, however, taxonomic identification based solely on morphological features can be misleading and unreliable. In contrast, DNA based identification provides a powerful and reliable method for taxonomic discrimination of fungi, it can be performed at any growth stages using parts of the fruit body, mono- and dikaryotic mycelia, or any other organic fungal. In the current study, two different molecular markers including Internal Transcribed Spacer (ITS) I and II and Intergenic Spacer (IGS) I were developed to identify the common cultivated edible mushroom species. Phylogenetic trees could clearly distinguish the analysed species by showing distinct clades. Species differentiations were re-confirmed by AMOVA analysis, nucleotide divergence, haplotyping and P values. Moreover, the designed primers could be employed in phylogenetic studies of other Basidiomycetes. Polymorphism occurred throughout the regions of interest due to insertion-deletion and point mutations, and differentiated the analysed families as well as genera. This study proved that the two developed molecular markers can be used for taxonomic identification of the common cultivated edible mushrooms in Basidiomycetes.

Item Type:	Conference or Workshop Item (Paper)
Funders:	Research grants PS291-2009C and 66-02-03-0074 from the Institute of Research Management and Monitoring (IPPP), University of Malaya
Uncontrolled Keywords:	Basidiomycetes, ITS, IGS, DNA barcode, taxonomic identification
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Mr Farhat Ahmadi Avin
Date Deposited:	22 Oct 2013 03:58
Last Modified:	11 Dec 2013 02:44
URI:	http://eprints.um.edu.my/id/eprint/8503

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