A simple and low-cost technique of DNA extraction from edible mushrooms examined by molecular phylogenetics

Avin, F.A. and Bhassu, S. and Vikineswary, S. (2013) A simple and low-cost technique of DNA extraction from edible mushrooms examined by molecular phylogenetics. Research on Crops, 14 (3). pp. 897-901. ISSN 0972-3226

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Abstract

The first and most important step of molecular techniques is to isolate the high quality and standard quantity of DNA. The DNA extracted using the recommended method could successfully amplify the regions of interest and demonstrated reliable results can be applied in other molecular assays. Moreover, the designed primers of ITS1-UM2 and ITS4-UM2 were perfectly matched with the species of Basidiomycetes, can be used in phylogenetic studies of other mushrooms. We here evaluated the quality and quantity of DNA using a spectrophotometer, showed reliable OD260/280, and concentration. The protocol is efficient, rapid, low-cost, and simple, needs low amount of sample, and requires minimum facilities. The standard yield in addition to the high quality of DNA will enable mycologists to establish molecular techniques easier. In the current study, the constructed phylogenetic tree based on the obtained sequences of Internal Transcribed Spacer (ITS) I and II regions distinctly classified the examined material.

Item Type: Article
Uncontrolled Keywords: Basidiomycetes, DNA isolation, Internal Transcribed Spacer (ITS), phylogenetic, spectrophotometer
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr Farhat Ahmadi Avin
Date Deposited: 03 Oct 2013 01:22
Last Modified: 03 Oct 2013 01:22
URI: http://eprints.um.edu.my/id/eprint/8439

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