Abdullah, Ramli and Khaironizam, M.Z. and Hashida, N.H. and Ashikin, M. (2013) Effects of freezing on sperm velocity in four different age groups of jermasia buck. Indian Journal of Animal Research, 47 (1). pp. 54-57. ISSN 0367-6722,
Full text not available from this repository.Abstract
The present study was undertaken to investigate the effects of freezing on sperm velocity in four different age groups (young: <1 year; mature: e <= 1.0-2.5 years; old: e <= 2.5-4.5 years and very old: e <= 4.5 years) of Jermasia buck. Tris Citric Acid Egg Yolk Extender (TCAYE) was added to semen samples and cryopreserved using a programmable freezer prior to plunging into liquid nitrogen (-196 degrees C). The fresh extended and frozen-thawed semen were subjected to computer automated semen analyzer (CASA) for sperm velocity evaluation. Fresh extended sperm in mature age group showed significantly highest values for average path velocity (VAP) 76.73 +/- 1.24 mu m/s, straight line velocity (VSL) 55.30 +/- 1.05 mu m/s, curvilinear velocity (VCL) 144.31 +/- 2.38 mu m/s, amplitude of lateral head displacement (ALH) 6.59 +/- 0.10 mu m and beat cross frequency (BCF) 39.59 +/- 0.36Hz as compared to young, old and very old age groups of buck (P<0.05). Significantly (P<0.05) higher values for VAP, VSL, VCL and ALH were observed in fresh as compared to frozen-thawed sperm in all age groups of buck. Thus, age of bucks should be considered as one of the factors that contribute to better sperm velocity which might affect fertilizability of the sperm. Optimizing the freezing technique is also essential in order to obtain satisfactory results after artificial insemination.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Uncontrolled Keywords: | Age, Buck Computer automated semen analyzer (casa), Goat, Semen, Freezing, Brown-norway rat, Motility parameters, Semen, Cryopreservation, Subpopulations, Spermatozoa, Frozen, Goat, Fertility, Analyzer |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Miss Malisa Diana |
| Date Deposited: | 30 Sep 2013 07:11 |
| Last Modified: | 28 Apr 2021 08:08 |
| URI: | http://eprints.um.edu.my/id/eprint/8433 |
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