Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages

Abdullah, R.B. and Khadijah, W.F.W. and Bahiyah, A.B.A. (2012) Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages. Journal of Animal and Veterinary Advances, 11 (2). pp. 200-206. ISSN 1680-5593

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Abstract

This study evaluated the efficiency of direct plunging and vitrification techniques in preserving mouse embryos at various preimplantation stages. Embryos were from superovulated ICR female mice. In direct plunging, embryos were equilibrated in EG (4.0 M), sucrose (0.25 M), BSA (4%), loaded into straw and plunged into LN(2). The straw was thawed in water bath (37 degrees C) and embryos were equilibrated in sucrose (0.5 M), BSA (4%). In vitrification, embryos were equilibrated with EG (7.5%), DMSO (7.5%) with EG (15%), DMSO (15%) and loaded onto cryoleaf. The cryoleaf was plunged into LN(2). Embryos were warned in sucrose (1.0 M), sucrose (0.5 M), washing solution and in modified WM medium. Frozen-thawed/vitrified-warmed embryos were cultured in modified WM medium in 5% CO(2) incubator (37 degrees C). Some embryos were transferred into pseudopregnant CBA/ca females for in vivo viability assessment. Vitrification gave higher survival rates than direct plunging technique at all developmental stages studied. No significant differences in the percentage of live-birth from direct plunging (22.40 +/- 4.40%), vitrification (29.80 +/- 9.00%) and control embryos (28.83 +/- 3.25%).

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Cryopreservation, Direct plunging, Embryo, Mouse, Vitrification, Malaysia, Mammalian embryos, Ethylene-glycol, Human oocytes, Cryopreservation, Survival, Frozen, Cryoprotectant, Equilibrium, Spindle, Sucrose
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 30 Sep 2013 03:19
Last Modified: 26 Dec 2014 04:53
URI: http://eprints.um.edu.my/id/eprint/8427

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