Effect of sperm insemination duration on in vitro fertilisation (IVF) performance in goats

Abdullah, R.B.; Wan Khadijah, W.E.; Tan, W.L. (2011) Effect of sperm insemination duration on in vitro fertilisation (IVF) performance in goats. Malaysian Journal of Science, 30 (2). pp. 105-111. ISSN 13943065

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Abstract

The objective of this study was to evaluate the effect of sperm insemination duration during in vitro fertilisation (IVF) on the cleavage rate of goat embryos. Goat oocytes were retrieved through the slicing of ovaries obtained from abattoir and laparoscopic oocyte pick-up (LOPU). For In vitro maturation (IVM), the collected cumulus oocyte complexes (COCs) were matured and then incubated overnight in CO 2(5%) incubator at 38.5 °C for 24-27 hr (abattoir source) or 18-21 hr (LOPU source). Oocytes were partially denuded and co-incubated with post-thawed sperm with concentration of 1×10 6 sperm/ml. After 8-14 hr or 1824 hr of insemination, in vitro culture (IVC) of presumptive zygotes were further incubated in CO 2 (5%) incubator at 38.5°C for preimplantation embryonic development. The cleavage of the embryos was observed and recorded daily under inverted microscope. The fertilisation rate with insemination duration of 8-14 hr (39.82%) was higher than that with 18-24 hr (33.59%); however, there was no significant difference (P>0.05) between 2 groups of insemination durations. There was significant (P<0.05) decrease as the embryo developmental stages increased. Generally, the embryo developmental rates of all stages for insemination durations of 8-14 hr were higher than that of 18-24 hr, which were 49.36 vs. 27.92%; 33.77 vs. 22.29%; 7.32 vs. 2.08% and 0.56 vs. 0.00% for 2-, 4-, 8-cell and morula, respectively. In conclusion, shorter insemination duration may be needed to improve the IVF performance in goats.

Item Type: Article
Creators:
  1. Abdullah, R.B.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Wan Khadijah, W.E.
  3. Tan, W.L.
Journal or Publication Title: Malaysian Journal of Science
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Embryo, Goat, Insemination duration, IVF
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 30 Sep 2013 10:58
Last Modified: 30 Sep 2013 10:58
URI: http://eprints.um.edu.my/id/eprint/8426

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