Prolonging the interval from ovarian hyperstimulation to laparoscopic ovum pick-up improves oocyte yield, quality, and developmental competence in goats

Abdullah, R.B. and Ng, S.C. and Chan, W.K. and Liow, S.L. and Wan-Khadijah, W.E. and Rahman, A. (2008) Prolonging the interval from ovarian hyperstimulation to laparoscopic ovum pick-up improves oocyte yield, quality, and developmental competence in goats. Theriogenology, 70 (5). pp. 765-771. ISSN 0093-691X

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Abstract

The objective was to evaluate the effect of the interval between ovarian hyperstimulation and laparoscopic ovum pick-Lip (LOPU) on quality and developmental competence of goat oocytes before and after in vitro maturation (IVM) and intracytoplasmic sperm injection (ICSI). Estrus was synchronized with an intravaginal insert containing 0.3 g progesterone (CIDR) for 10 d, combined with a luteolytic treatment of 125 mu g cloprostenol 36 It prior to CUP removal. Ovaries were hyperstimulated with 70 mg FSH and 500 IU hCG given im 36, 60, or 72 It prior to LOPU (n = 15, 16, and 7 does, respectively). For these groups, oocyte retrieval rates (mean +/- S.E.M.) were 24.7 +/- 2.9, 54.5 +/- 4.7, and 82.8 +/- 4.60%; (P < 0.001), and the proportions of cumulus-oocyte complexes (COC) with more than five layers of cumulus cells were 29.7 +/- 8.3, 37.6 +/- 6.9, and 37.3 +/- 7.0% (P < 0.001). The proportion of IVM oocytes was highest at 72 h (82.1 +/- 2.8%; P < 0.05), with no significant difference between 36 and 60 h (57.3 +/- 8.9% and 69.0 +/- 8.4%). Cleavage rates of ICSI embryos were 4.2 +/- 4.2, 70.9 +/- 8.4, and 78.9 +/- 8.2% with LOPU 36, 60, and 72 It post FSH/hCG (P < 0.01), with a lower proportion of Grade-A embryos (P < 0.05) following LOPU at 36 h compared to 60 and 72 h (29.7 +/- 8.3%, 37.6 +/- 6.9%, and 37.3 +/- 7.0%). In summary, a prolonged interval from FSH/hCG to LOPU improved oocyte retrieval rate and oocyte quality. Therefore, under the present conditions, LOPU 60 or 72 It after FSH/hCG optimized yields of good-quality oocytes for IVM and embryo production in goats. (c) 2008 Elsevier Inc. All rights reserved.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Oocyte, Embryo, Laparoscopic ovum pick-up, Goat, Developmental, Competence, Vitro embryo production, Human chorionic-gonadotropin, Culture in-vitro, Follicle size, Subordinate follicles, Transgenic goats, Bovine oocytes, Fertilization, Technologies, Season
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 10 Oct 2013 01:44
Last Modified: 10 Oct 2013 01:44
URI: http://eprints.um.edu.my/id/eprint/8392

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