The effects of biopsy and cryopreservation by vitrification on the in vitro development of eight-cell mouse embryos

Abdullah, Ramli and Mohamed Said, M.S. and Nor Ashikin, M.N.K. and Dzulsuhaimi, D. and Rajikin, M.H. and Mohamed, Z. and Faizah, S. (2006) The effects of biopsy and cryopreservation by vitrification on the in vitro development of eight-cell mouse embryos. Biomedical Research, 17 (1). pp. 61-66. ISSN 0970938X

Full text not available from this repository. (Request a copy)
Official URL: http://www.indmedica.com/journals.php?journalid=12...

Abstract

In vitro and in vivo development of cryopreserved and non-cryopreserved biopsied 8-cell mouse embryos were examined in this study. It was observed that intact and biopsied embryos could successfully be cryopreserved by using the blastomere biopsy and vitrification procedure. However, in vitro survivability of the embryos declined steadily with advancing developmental stages, irrespective of the treatments used. Overall, one-blastomere biopsy without cryopreservation yielded the highest rate of development to morula (96.1%), expanded blastocyst (84.3%) and hatched blastocyst (74.5%), respectively. When the effect of cryopreservation was taken into account, two-blastomere biopsy did not differ significantly from that of the one-blastomere biopsy in terms of development to the expanded and hatched blastocyst stages. The results of our study suggest that the two-blastomere biopsy with cryopreservation is a better option in the current set-up.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Cryopreservation, Embryo biopsy, Mouse embryo, Preimplantation genetic diagnosis, article, biopsy, blastocyst, blastomere, developmental stage, embryo, embryo culture, in vitro study, morula, mouse, nonhuman, survival rate, vitrification
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 10 Oct 2013 01:48
Last Modified: 28 Apr 2021 07:40
URI: http://eprints.um.edu.my/id/eprint/8389

Actions (login required)

View Item View Item