Developmental competence of transported in-vitro matured macaque oocytes

Abdullah, Ramli and Ng, S.C. and Chen, N. and Liow, S.L. and Tong, G.Q. and Embong, W.K.W. and Yip, W.Y. and Tan, L.G. (2006) Developmental competence of transported in-vitro matured macaque oocytes. Reproductive BioMedicine Online, 12 (1). pp. 50-59. ISSN 14726483

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Abstract

This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing pre-equilibrated IVM medium and transported for 5 h in a dry portable 37°C incubator without CO 2 supplement. Meiotic spindle was observed at 36-38- h post-HCG by polarized microscopy in 72 and 84.5% of mature oocytes collected at 12- and 24- h post-HCG ooeyte retrieval intervals respectively. However, abnormal spindle formations were detected in some IVM oocytes by confocal microscopy. The IVM oocytes were also randomly selected for (i) intracytoplasmic injection with frozen-thawed epididymal M. fascicularis spermatozoa and (ii) nuclear transfer (NT) with fresh M. fascicularis cumulus cells. Embryonic development of sperm-injected embryos was not affected by the 12- and 24- h post-HCG oocyte retrieval intervals (22.5 versus 27.9% respectively). However, embryonic development of NT embryos was significantly affected by the 12- h post-HCG oocyte retrieval interval (4.5 versus 31.7% respectively; P < 0.01). In conclusion, IVM of monkey oocytes in a dry portable incubator for 5 h did not affect the maturation rate. However, the ability of primate oocytes to develop after somatic cell nuclear transfer was affected by oocyte retrieval time post-HCG.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: In-vitro maturation, Intracytoplasmic sperm injection, Monkey, Nuclear transfer, Oocyte transport, Chorionic gonadotropin, Recombinant follitropin, Triptorelin, Animal cell, Animal experiment, Article, Cell nucleus transplantation, Confocal microscopy, Embryo, Embryo development, In vitro study, Macaca, Male, Meiosis, Nonhuman, Oocyte development, Oocyte maturation, Ovulation induction, Somatic cell, Spermatozoon, Animals, newborn/*blood/immunology, Cell culture techniques, Female, Macaca fascicularis, Microscopy, confocal, Mitotic spindle apparatus, Oocytes, Specimen handling, Sperm injections, intracytoplasmic, Time factors, Zygote
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Miss Malisa Diana
Date Deposited: 24 Oct 2013 02:10
Last Modified: 28 Apr 2021 08:01
URI: http://eprints.um.edu.my/id/eprint/8387

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