Novel antiviral activity of baicalein against dengue virus

Zandi, Keivan and Teoh, B.T. and Sam, S.S. and Wong, P.F. and Mustafa, M.R. and AbuBakar, Sazaly (2012) Novel antiviral activity of baicalein against dengue virus. BMC Complementary and Alternative Medicine, 12 (214).

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Abstract

Background: Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits. Methods: In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR. Results: Baicalein inhibited DENV-2 replication in Vero cells with IC50 = 6.46 mu g/mL and SI = 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 mu g/mL and SI = 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC50 = 1.55 mu g/mL and showed anti-adsorption effect with IC50 = 7.14 mu g/mL. Conclusions: Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV.

Item Type: Article
Additional Information: Times Cited: 0 Zandi, Keivan Teoh, Boon-Teong Sam, Sing-Sin Wong, Pooi-Fong Mustafa, Mohd Rais AbuBakar, Sazaly
Uncontrolled Keywords: Infectious disease, Flavivirus, Antiviral, Dengue, Flavonoid, Baicalein
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 02 Sep 2013 02:27
Last Modified: 13 Feb 2019 08:32
URI: http://eprints.um.edu.my/id/eprint/8320

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