Bioactivity-guided fractionation of the lipoxygenase and cyclooxygenase inhibiting constituents from Chisocheton polyandrus Merr

Chan, K.Y. and Mohamad, K. and Ooi, A.J.A. and Imiyabir, Z. and Chung, L.Y. (2012) Bioactivity-guided fractionation of the lipoxygenase and cyclooxygenase inhibiting constituents from Chisocheton polyandrus Merr. Fitoterapia, 83 (5). pp. 961-967. ISSN 0367-326X, DOI https://doi.org/10.1016/j.fitote.2012.04.018.

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Abstract

Lipoxygenase (LOX)-inhibiting compounds from the leaves of Chisocheton polyandrus Merr. were isolated in this study using a bioactivity-guided fractionation technique. Two dammarane triterpenoids, dammara-20,24-dien-3-one (1) (IC50 = 0.69 +/- 0.07 mu M) and 24-hydroxydammara-20,25-dien-3-one (2) (IC50 = 1.11 +/- 0.38 mu M), were isolated and identified based on the soybean LOX assay. Dammara-20,24-dien-3-one (1) exhibited dual inhibition of both human 5-LOX (IC50 = 24.27 +/- 2.92 mu M) and cyclooxygenase-2 (COX-2) (IC50 = 3.17 +/- 0.90 mu M), whereas 24-hydroxydammara-20,25-dien-3-one (2) did not exhibit any significant inhibitory effects. This report is the first to detail the inhibition of LOX and COX by both C. polyandrus and its isolated compounds. (C) 2012 Elsevier B.V. All rights reserved.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Chan, Kit Yee Mohamad, Khalit Ooi, Audrey J. A. Imiyabir, Zamrie Chung, Lip Yong
Uncontrolled Keywords:	Chisocheton polyandrus; Dammarane triterpenoid;Bioactivity-guided isolation;Lipoxygenase; Cyclooxygenase
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	22 Jul 2013 00:32
Last Modified:	22 Jul 2013 00:32
URI:	http://eprints.um.edu.my/id/eprint/8153

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