Lee, S.Y. and Wee, A.S. and Lim, C.K. and Abbas, A.A. and Selvaratnam, L. and Merican, A.M. and Ahmad, T.S. and Kamarul, Tunku (2013) Supermacroporous poly (vinyl alcohol)-carboxylmethyl chitosan-poly (ethylene glycol) scaffold: an in vitro and in vivo pre-assessments for cartilage tissue engineering. Journal of Materials Science: Materials in Medicine. pp. 1-10. ISSN 0957-4530, DOI https://doi.org/10.1007/s10856-013-4907-4.
Full text not available from this repository.Abstract
This study aims to pre-assess the in vitro and in vivo biocompatibility of poly(vinyl alcohol)-carboxylmethyl-chitosan-poly(ethylene glycol) (PCP) scaffold. PCP was lyophilised to create supermacroporous structures. 3-(4, 5-dimethyl-thiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and immunohistochemistry (IHC) were used to evaluate the effectiveness of PCP scaffolds for chondrocytes attachment and proliferation. The ultrastructural was assessed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Extracellular matrix (ECM) formation was evaluated using collagen type-II staining, glycosaminoglycan (GAG) and collagen assays. Histological analysis was conducted on 3-week implanted Sprague�Dawley rats. The MTT, IHC, SEM and TEM analyses confirm that PCP scaffolds promoted cell attachment and proliferation in vitro. The chondrocyte-PCP constructs secreted GAG and collagen type-II, both increased significantly from day-14 to day-28 (P < 0.05). PCP scaffolds did not elicit any adverse effects on the host tissue, but were partially degraded. These results suggest that supermacroporous PCP is a biocompatible scaffold for clinical applications.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Subjects: | R Medicine |
| Depositing User: | Ms Haslinda Lahuddin |
| Date Deposited: | 03 Jul 2013 08:59 |
| Last Modified: | 10 Oct 2018 08:45 |
| URI: | http://eprints.um.edu.my/id/eprint/8116 |
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