Tan, K.W. and Ng, C.H. and Seng, H.L. and Von, S.T. and Maah, M.J. and Ng, S.W. and Rahman, R. and Caracelli, I. (2010) Crystal structure, DNA binding studies, Nucleolytic property and Topoisomerase I Inhibition of zinc complex with 1,10-Phenanthroline and 3-Methyl-Picolinic Acid. Biometals, 23 (1). pp. 99-118. ISSN 0966-0844, DOI https://doi.org/10.1007/s10534-009-9271-y.
Full text not available from this repository.Abstract
Crystal structure analysis of the zinc complex establishes it as a distorted octahedral complex, bis(3-methylpicolinato-kappa(2) N,O)(2)(1,10-phenanthroline-kappa(2) N,N)-zinc(II) pentahydrate, [Zn(3-Me-pic)(2)(phen)]center dot 5H(2)O. The trans-configuration of carbonyl oxygen atoms of the carboxylate moieties and orientation of the two planar picolinate ligands above and before the phen ligand plane seems to confer DNA sequence recognition to the complex. It cannot cleave DNA under hydrolytic condition but can slightly be activated by hydrogen peroxide or sodium ascorbate. Circular Dichroism and Fluorescence spectroscopic analysis of its interaction with various duplex polynucleotides reveals its binding mode as mainly intercalation. It shows distinct DNA sequence binding selectivity and the order of decreasing selectivity is ATAT > AATT > CGCG. Docking studies lead to the same conclusion on this sequence selectivity. It binds strongly with G-quadruplex with human tolemeric sequence 5'-AG(3)(T(2)AG(3))(3)-3', can inhibit topoisomerase I efficiently and is cytotoxic against MCF-7 cell line.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Chemistry, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science > Department of Chemistry |
| Depositing User: | Miss Malisa Diana |
| Date Deposited: | 05 Jul 2013 08:21 |
| Last Modified: | 21 Aug 2019 09:19 |
| URI: | http://eprints.um.edu.my/id/eprint/8026 |
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