Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin

Phuah, N.H. and In, L.L.A. and Azmi, M.N. and Ibrahim, H. and Awang, Khalijah and Nagoor, Noor Hasima (2013) Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin. Reproductive Sciences, 20 (5). pp. 567-578. ISSN 1933-7191

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Abstract

The aims of this study were to investigate the combined effects of a natural compound 1²S-1²-acetoxychavicol acetate (ACA) with cisplatin (CDDP) on HPV-positive human cervical carcinoma cell lines (Ca Ski - low cisplatin sensitivity and HeLa - high cisplatin sensitivity), and to identify microRNAs (miRNAs) modulated in response toward ACA and/or CDDP. It was revealed that both ACA and CDDP induced dose- and time-dependent cytotoxicity when used as a stand-alone agent, while synergistic effects were observed when used in combination with a combination index (CI) value of 0.74 ± 0.01 and 0.85 ± 0.01 in Ca Ski and HeLa cells, respectively. A total of 25 miRNAs were found to be significantly differentially expressed in response to ACA and/or CDDP. These include hsa-miR-138, hsa-miR-210, and hsa-miR-744 with predicted gene targets involved in signaling pathways regulating apoptosis and cell cycle progression. In conclusion, ACA acts as a chemosensitizer which synergistically potentiates the cytotoxic effect of CDDP in cervical cancer cells. The altered miRNA expression upon administration of ACA and/or CDDP suggests that miRNAs play an important role in anticancer drug responses, which can be manipulated for therapeutic purposes. © The Author(s) 2012.

Item Type: Article
Uncontrolled Keywords: 1â'-acetoxychavicol acetate, cervical cancer, cisplatin, combination chemotherapy, microRNA,
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: miss munirah saadom
Date Deposited: 15 Jul 2013 00:45
Last Modified: 01 Mar 2019 09:22
URI: http://eprints.um.edu.my/id/eprint/7784

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