Distribution of Candida in the oral cavity and its differentiation based on the internally transcribed spacer (ITS) regions of rDNA

Zahir, R.A. and Himratul-Aznita, W.H. (2013) Distribution of Candida in the oral cavity and its differentiation based on the internally transcribed spacer (ITS) regions of rDNA. Yeast, 30 (1). pp. 13-23. ISSN 0749-503X

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Abstract

This study aimed to determine the distribution of Candida species in the oral cavity and differentiate the species based on PCR amplification, including HinfI and MspI digestion, in order to assess the effectiveness of using the rDNA region for species identification. Samples from saliva as well as palate, tongue and cheek mucosa surfaces were collected from 45 individuals, consisting of three groups: periodontal disease patients; denture-wearers; and the control group. The samples were serially diluted, spread on BHI and YPD agar plates and scored for colony-forming units (CFUs). Fifteen random candidal colonies were isolated and subjected to genomic DNA extraction, based on glass beads disruption. Four primers were used to amplify regions in the rDNA, and the ITSI-5.8S-ITSII PCR product was digested by HinfI and MspI restriction enzymes. The microbial loads on all sites of the denture-wearers were found to be significantly higher than control, while in the periodontal disease group only the microbial loads on the tongue were significantly higher than control. Meanwhile, there was no significant difference at other sites. The restriction fragment lengths of the clinical samples were compared to those of seven control species, allowing the differentiation of all seven species and the identification of 14 species from the clinical samples. The MspI restriction digest was not able to distinguish between C. albicans and C. dubliniensis, whereas the HinfI digest could not distinguish between C. tropicalis and C. parapsilosis. It was concluded that PCRRFLP of the candidal rDNA region has potential for species identification. This study demonstrates the potential use of candidal rDNA as a means for identifying Candida species, based on genotype. The results also indicate the possibility of constructing genetic probes that target specific restriction fragments in the ITSI-5.8S-ITSII region, enabling swift and precise identification of Candida species. Copyright (c) 2012 John Wiley & Sons, Ltd.

Item Type: Article
Additional Information: ISI Document Delivery No.: 076WJ Times Cited: 0 Cited Reference Count: 30 Cited References: Akpan A, 2002, POSTGRAD MED J, V78, P455, DOI 10.1136/pmj.78.922.455 ARENDORF TM, 1980, ARCH ORAL BIOL, V25, P1, DOI 10.1016/0003-9969(80)90147-8 Cannon RD, 1999, CRIT REV ORAL BIOL M, V10, P359 Dale DC, 2000, BLOOD, V96, P2317 EDGERTON M, 1993, INFECT IMMUN, V61, P2644 Ellepola ANB, 2003, ORAL MICROBIOL IMMUN, V18, P379, DOI 10.1046/j.0902-0055.2003.00103.x Fujita SI, 2001, J CLIN MICROBIOL, V39, P3617, DOI 10.1128/JCM.39.10.3617-3622.2001 GIBBONS RJ, 1989, J DENT RES, V68, P750, DOI 10.1177/00220345890680050101 HARRIS D, 1992, J PHARM SCI, V81, P1, DOI 10.1002/jps.2600810102 HEDGES S, 1992, INFECT IMMUN, V60, P1295 Iwen PC, 2002, MED MYCOL, V40, P87, DOI 10.1080/714031073 Kumar M, 2005, J CLIN MICROBIOL, V43, P662, DOI 10.1128/JCM.43.2.662-668.2005 LAMSTER IB, 1992, J PERIODONTOL, V63, P1117 Lamster IB, 2003, J PERIODONTOL, V74, P353, DOI 10.1902/jop.2003.74.3.353 Li Y, 2005, J DENT RES, V84, P559 Mirhendi Hossein, 2006, Nihon Ishinkin Gakkai Zasshi, V47, P225, DOI 10.3314/jjmm.47.225 Niimi M, 1999, ELECTROPHORESIS, V20, P2299, DOI 10.1002/(SICI)1522-2683(19990801)20:11<2299::AID-ELPS2299>3.3.CO;2-Z Peterson D E, 1992, Clin Geriatr Med, V8, P513 Pincus DH, 1999, J CLIN MICROBIOL, V37, P3533 Pires FR, 2002, J ORAL REHABIL, V29, P1115, DOI 10.1046/j.1365-2842.2002.00947.x Samaranayake L. P., 1990, Oral candidosis., P124 SCULLY C, 1994, BRIT MED J, V308, P217 Shokohi T, 2010, INDIAN J MED MICROBI, V28, P147, DOI 10.4103/0255-0857.62493 Shulman JD, 2005, J ORAL PATHOL MED, V34, P340, DOI 10.1111/j.1600-0714.2005.00287.x Takahashi N, 2005, INT C SER, V1284, P103, DOI 10.1016/j.ics.2005.06.071 Tenovuo J, 1992, J Biol Buccale, V20, P85 Theilade E., 1990, HUMAN MICROBIAL ECOL, P2 Turenne CY, 1999, J CLIN MICROBIOL, V37, P1846 VANDERVELDEN U, 1986, J CLIN PERIODONTOL, V13, P243 Zadik Y, 2010, ORAL DIS, V16, P172, DOI 10.1111/j.1601-0825.2009.01618.x Zahir, R. A. Himratul-Aznita, W. H. University of Malaya FP011/2006A, FS010/2007A, PS324/2009A This study was financially supported by the University of Malaya research grant (Grant Nos FP011/2006A, FS010/2007A and PS324/2009A). We are also grateful for the assistance provided by Dr Nor Adinar Baharuddin and Dr Ros Anita Omar in the clinical sample collection undertaken for this study. Wiley-blackwell Hoboken
Uncontrolled Keywords: Candida albicans C tropicalis C krusei C parapsilosis C dubliniensis C glabrata C lusitaniae rapid identification denture stomatitis albicans electrophoresis colonization dubliniensis prevalence adhesion saliva fungi
Subjects: R Medicine > RK Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 16 Jul 2013 03:30
Last Modified: 30 May 2017 12:22
URI: http://eprints.um.edu.my/id/eprint/7737

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