Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines

Alabsi, A.M. and Ali, R. and Ali, A.M. and Al-Dubai, S.A.R. and Harun, H. and Abu Kasim, N.H. and Alsalahi, A. (2012) Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines. Asian Pacific Journal of Cancer Prevention, 13 (10). pp. 5131-5136. ISSN 1513-7368

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Abstract

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.

Item Type: Article
Additional Information: Alabsi, Aied M. Ali, Rola Ali, Abdul Manaf Al-Dubai, Sami Abdo Radman Harun, Hazlan Abu Kasim, Noor H. Alsalahi, Abdulsamad
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 16 Jul 2013 03:28
Last Modified: 16 Jul 2013 03:28
URI: http://eprints.um.edu.my/id/eprint/7731

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