Light-scattering and turbidimetric detection of silica colloids in size-exclusion chromatography

Aspanut, Z. and Takeuchi, T. and Lim, L.W. and Yamada, T. (2008) Light-scattering and turbidimetric detection of silica colloids in size-exclusion chromatography. Analytical and Bioanalytical Chemistry, 391 (1). pp. 353-359. ISSN 1618-2642

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Abstract

Silica colloids were separated by size-exclusion chromatography and monitored by fluorimetric and UV detection. In the former means of detection, silica colloids were visualized by light-scattering. The signal intensity based on the light scattering increased with increasing size of the silica colloids. The maximum intensity was observed at excitation wavelengths around 270-290nm. In UV detection, silica colloids were visualized based on turbidimetry, and the signal intensity also increased with increasing size of the silica colloids and with decreasing detection wavelength. The signal intensities for both light-scattering and turbidimetric detection were a linear function of the concentration of the silica colloids. The detection limit at S/N=3 for 78-nm colloids was 0.06 ppm for light-scattering detection whereas the LOD was 2.3 ppm for UV detection. Effects of mobile phase conditions and flow rate on resolution and peak shape were examined. Use of phosphate buffer allowed the separation of silica colloids of different sizes in size-exclusion chromatography.

Item Type: Article
Additional Information: Department of Physics, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Silica colloids; size-exclusion chromatography; fluorimetric and UV detection; signal intensity; maximum intensity; turbidimetry; mobile phase conditions; flow rate on resolution
Subjects: Q Science > QC Physics
Divisions: Faculty of Science > Dept of Physics
Depositing User: Miss Malisa Diana
Date Deposited: 17 Jul 2013 06:58
Last Modified: 16 Dec 2014 06:54
URI: http://eprints.um.edu.my/id/eprint/7473

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