A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles

Tanaka, S. and Katsura, S. and Hatsukade, Y. and Toriyabe, C. and Kurita, H. and Aspanut, Z. (2005) A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles. IEEE Transactions on Applied Superconductivity, 15 (2). pp. 664-667. ISSN 1051-8223

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Abstract

A high Tc SQUID system for biological molecules (DNA) detection is developed. This system is based on a hybridization process. Two strands in a DNA molecule are held together by hydrogen bonds between base pairs like a ladder. The two strands are referred to as being complementary each other. HPVB 33 (Human Papillomavirus Probes 33) was prepared as a DNA. One strand (Sample DNA) was labeled with Fe3O4 ultra-small magnetic particles and the other (probe DNA) was anchored on a glass slide. Then they were hybridized each other on the slide. After washing the excess sample DNA, the hybridized DNA was evaluated in the presence of excitation ac field by high Tc SQUID. The signal was initially proportional to the concentration of the sample DNA and then saturated. It means that the hybridization occurred successfully between the sample DNA and the probe DNA.

Item Type: Article
Additional Information: Department of Physics, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: hybridization; magnetic detection; medical applications; small particles; squid; molecules
Subjects: Q Science > QC Physics
Divisions: Faculty of Science > Dept of Physics
Depositing User: Miss Malisa Diana
Date Deposited: 17 Jul 2013 06:20
Last Modified: 08 Jul 2017 03:56
URI: http://eprints.um.edu.my/id/eprint/7466

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