Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library

Yuen, Hawk Leong and Shamala, Devi and Thong, Kwai Lin (2008) Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library. Journal of Infection in Developing Countries, 2 (4). pp. 313-23. ISSN 2036-6590

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Official URL: https://doi.org/10.3855/jidc.228

Abstract

BACKGROUND: Heat shock proteins (HSPs) are known to be involved in the pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever. The objective of this study was to apply a phage display library to identify mimotopes of two HSPs, HSP90 and DnaK in S. Typhi. METHODOLOGY: A 12-mer random peptide library expressed on the surface of the filamentous phage, M13, was used to select the mimotopes of two S. Typhi heat shock proteins by biopanning with monoclonal antibodies (mAbs), DnaK and HSP90. The immunogenicity of the selected peptides was determined through binding affinity with polyclonal antibodies from pooled typhoid-confirmed patients' sera and purified HSPs mAb using Western blotting and ELISA. RESULTS: Five rounds of biopanning resulted in enrichment of phage clones expressing the binding motifs TDxSTRP and FPSHYWLYPPPT, respectively. The selected peptides showed strong immunoreactivity with patients' sera. Thus, monoclonal antibodies against HSP and patient sera can select common mimotopes from the random peptide library. CONCLUSION: These findings may provide fundamental information for further studies on diagnostic application or vaccine design against this aetiologic agent of typhoid fever.

Item Type: Article
Additional Information: Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, Malaysia
Uncontrolled Keywords: Phage Display; Heat Shock Proteins; S. Typhi
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
R Medicine > RB Pathology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 16 Apr 2011 02:49
Last Modified: 15 Oct 2018 03:00
URI: http://eprints.um.edu.my/id/eprint/727

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