Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization

Wong, E.H.; Subramaniam, G.; Navaratnam, P.; Sekaran, S.D. (2007) Rapid detection of non-enterobacteriaceae directly from positive blood culture using fluorescent in situ hybridization. Indian journal of medical microbiology, 25 (4). pp. 391-4. ISSN 0255-0857

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Abstract

Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes specific for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly specific. Sensitivity testing showed that the probes were able to detect as low as 10 3 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.

Item Type: Article
Journal or Publication Title: Indian journal of medical microbiology
Additional Information: Department of Medical Microbiology, Faculty of Medicine, University Malaya, 50603, Kuala Lumpur, Malaysia.
Uncontrolled Keywords: Acinetobacter/genetics; Acinetobacter/isolation & purification
Subjects: R Medicine > RC Internal medicine
Divisions: Faculty of Medicine > School of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 16 Apr 2011 10:41
Last Modified: 27 Oct 2014 14:15
URI: http://eprints.um.edu.my/id/eprint/723

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