Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR

Muniandy, S. and Yaiw, K.C. and Chook, J.B. and Rayappan, A.V. and Wong, K.T. and Ismail, I.S. and Qvist, R. (2008) Quantification of Zonula Occludens-1 mRNA expression in cultured bovine retinal endothelial cells, using SYBR green real-time PCR. Biomedical Research-India, 19 (2). pp. 117-124. ISSN 0970-938X,

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Abstract

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10(-7)M to 10(-9) M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohistochemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Times Cited: 0 Muniandy, Sekaran Yaiw, Koon Chu Chook, Jack Bee Rayappan, Arokiasamy Vinscent Wong, Kum Thong Ismail, Ikram Shah Qvist, Rajes
Uncontrolled Keywords:	Diabetes; Bovine retinal endothelial cells; Zonula Occludens-1; Real time reverse transcription poly-merase chain reaction
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms Haslinda Lahuddin
Date Deposited:	12 Jul 2013 01:16
Last Modified:	28 Oct 2014 05:53
URI:	http://eprints.um.edu.my/id/eprint/7220

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