Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda)

Ng, C.H. and Ng, S.W. and Yamin, B.M. and Rahman, R.N. and Tan, Kong Wai and Kong, C.W. and Ong, H.K.A. (2006) Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda). Polyhedron, 25 (16). pp. 3118-3126. ISSN 0277-5387

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Abstract

M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV-visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N'-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na(2)H(2)EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change. (C) 2006 Elsevier Ltd. All rights reserved.

Item Type: Article
Additional Information: Department of Chemistry, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: (N,N′-ethylenediaminediacetato)metal(II); DNA cleavage; Crystal structure; Nucleolytic efficiency; Hydrogen peroxide
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Dept of Chemistry
Depositing User: Miss Malisa Diana
Date Deposited: 09 Jul 2013 07:30
Last Modified: 27 Sep 2019 08:58
URI: http://eprints.um.edu.my/id/eprint/6949

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