Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris.

Lau, Y.L.; Shamilah, H.; Fong, M.Y. (2006) Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. Tropical Biomedicine, 23 (2). pp. 186-93. ISSN 0127-5720

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Abstract

A truncated form of surface antigen 2 (SAG2) of the protozoan parasite Toxoplasma gondii was cloned and expressed in the methylotrophic yeast Pichia pastoris. This recombinant antigen, designated as recSAG2-N, contained only the N-terminal half of the native SAG2. The recSAG2-N was secreted by the Pichia pastoris into the culture supernatant, and it was harvested by using the trichloroacetic acid precipitation method. Specificity of recSAG2-N was evaluated in western blot assays. Fifty human serum samples, including 32 from confirmed cases of toxoplasmosis, were tested. Results from the assays showed that recSAG2-N reacted with sera from the toxoplasmosis cases only. In vivo experiments showed that serum from mice which received recSAG2-N reacted with the native SAG2 of T. gondii.

Item Type: Article
Creators:
  1. Lau, Y.L.
  2. Shamilah, H.
  3. Fong, M.Y.
Journal or Publication Title: Tropical Biomedicine
Additional Information: Department of Parasitology, Faculty of Medicine, University of Malaya
Uncontrolled Keywords: Amino Acid Sequence; Animals; Antibodies, Protozoan/blood;
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Medicine
Depositing User: Mr. Faizal Hamzah
Date Deposited: 24 Mar 2011 12:31
Last Modified: 21 Oct 2014 12:37
URI: http://eprints.um.edu.my/id/eprint/622

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